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汪维鹏

发布者:汪维鹏发布时间:2018-11-04浏览次数:4031

 

 

 

姓  名:汪维鹏

       称:教   授、博士生导师

       务副院长

联系电话:0512-65883761

电子邮箱:wangweipeng@suda.edu.cn

办公地址:云轩楼1339

 

 个人简介:

一、学习工作经历

1、学习经历:

1997.92002.7 中国药科大学,中药药理学,本科

2002.92007.6 中国药科大学,药物分析学,博士

2、工作经历:

2007.8-至今     苏州大学药学院,药物分析系,讲师、副教授、教授

2008.9-2011.8 苏州大学医学生物技术研究所,博士后

2013.6-2014.6 美国加州大学戴维斯分校,博士后

 

二、社会兼职

(1)江苏省执业药师协会,常务理事

(2)中国高等教育学会医学教育专业委员会药学教育研究会,理事

(3)江苏省高等教育学会医学教育专业委员会,理事

(4)中华医学会医学教育分会药学教育学组,组员

(5)江苏省药学会药物基因组学专业委员会,委员

(6)中国药理学会药物代谢专业委员会青年委员会,常务委员


三、研究方向

1)肿瘤免疫与治疗

2)肿瘤药物基因组学 

(3)肿瘤药物代谢动力学

 

四、主要研究成果


1、论文*通讯作者)


k  肿瘤免疫与治疗

(1)Yang M#, Liu XC#, Meng FY, Zhang YW, Wang MM, Chen YS, Guo XQ, Chen WC*, Wang WP*. The rs7911488-T allele promotes the growth and metastasis of colorectal cancer through modulating miR-1307/PRRX1. Cell Death Dis. 2020, 11: 651.

(2)Zhang CX#, Chen YS#, Li FC#, Yang M, Meng FY, Zhang YW, Chen WC*, Wang WP*. B7-H3 is spliced by SRSF3 in colorectal cancer. Cancer Immunol Immunother. 2020 Jul 27. doi: 10.1007/s00262-020-02683-9.

(3)Yang JJ, Wang ZM, Liu S, Wang WP*, Zhang HJ, Gui CS*.  Functional Characterization Reveals the Significance of Rare Coding Variations in Human Organic Anion Transporting Polypeptide 2B1 (SLCO2B1). Mol Pharm. 2020, 17(10):3966-3978.

(4)Kong JH, Wang WP*. A systemic review on the regulatory roles of miR-34a in gastrointestinal cancer. Onco Targets Ther 2020, 13: 2855-2872.

(5)Wang JY, Wang WP*. B7-H4, a promising target for immunotherapy. Cell Immunol 2020, 347: 104008.

(6)李静梅,汪维鹏*. B7/CD28家族分子的糖基化研究进展. 现代免疫学2020402):153-157.

(7)Tao LH#, Zhou XR#, Li FC, Chen Q, Meng FY, Mao Y, Li R, Hua D, Zhang HJ, Wang WP*, Chen WC*. A polymorphism in the promoter region of PD-L1 serves as a binding-site for SP1 and is associated with PD-L1 overexpression and increased occurrence of gastric cancer. Cancer Immunol Immunother 2017, 66(3): 309-318.

(8)Zhou XR#, Mao Y#, Zhu JJ, Meng FY, Chen Q, Tao LH, Li R, Fu FQ, Liu CP, Hu YJ, Wang WP*, Zhang HJ, Hua D, Chen WC, Zhang XG. TGF-β1 promotes colorectal cancer immune escape by elevating B7-H3 and B7-H4 via the miR-155/miR-143 axis. Oncotarget 2016, 7(41): 67196-67211.

(9)Zhao Y, Tu MJ, Wang WP, Qiu JX, Yu AX*, Yu AM*. Genetically engineered pre-microRNA-34a prodrug suppresses orthotopic osteosarcoma xenograft tumor growth via the induction of apoptosis and cell cycle arrest. Sci Rep 2016, 6: 26611.

(10)    Wang WP#, Ho PY#, Chen QX#, Addepalli B, Limbach PA, Li MM, Wu WJ, Jilek JL, Qiu JX, Zhang HJ, Li T, Wun T, White RD, Lam KS, Yu AM*. Bioengineering novel chimeric microRNA-34a for prodrug cancer therapy: High-yield expression and purification, and structural and functional characterization. J Pharmacol Exp Ther 2015, 354(2): 131-141.

(11)    Tang R#, Qi Q#, Wu R, Zhou X, Wu D, Zhou H, Mao Y, Li R, Liu C, Wang L, Chen W, Hua D, Zhang H, Wang WP*. The polymorphic terminal-loop of pre-miR-1307 binding with MBNL1 contributes to colorectal carcinogenesis via interference with Dicer1 recruitment. Carcinogenesis 2015, 36(8): 867-875.

(12)    Wu D#, Tang R#, Qi Q, Zhou X, Zhou H, Mao Y, Li R, Liu C, Wang WP*, Hua D*, Zhang X. Five functional polymorphisms of B7/CD28 co-signaling molecules alter susceptibility to colorectal cancer. Cell Immunol2015, 293(1): 41-48.

(13)    Zhao Y, Tu MJ, Yu YF, Wang WP, Chen QX, Qiu JX, Yu AX*, Yu AM*. Combination therapy with bioengineered miR-34a prodrug and doxorubicin synergistically suppresses osteosarcoma growth. Biochem Pharmacol 2015, 98(4): 602-613.

(14)    Zhou H, Hu YU, Wang W, Mao Y, Zhu J, Zhou B, Sun J, Zhang X*. Expression of Oct-4 is significantly associated with the development and prognosis of colorectal cancer. Oncol Lett 2015, 10(2): 691-696.

(15)    Zhu J#, Chen L#, Zou L, Yang P, Wu R, Mao Y, Zhou H, Li R, Wang K, Wang WP*, Hua D*, Zhang X. MiR-20b, -21, and -130b inhibit PTEN expression resulting in B7-H1 over-expression in advanced colorectal cancer. Hum Immunol2014, 75(4): 348-353.

(16)    Wu R, Li F, Zhu J, Tang R, Qi Q, Zhou X, Li R, Wang W, Hua D*, Chen W*. A functional variant at miR-132-3p, miR-212-3p, and miR-361-5p binding site in CD80 gene alters susceptibility to gastric cancer in a Chinese Han population. Med Oncol 2014, 31(8): 60.

(17)    Zhang L#, Wu H#, Lu D, Li G, Sun C, Song H, Li J, Zhai T, Huang L, Hou C, Wang WP, Zhou B, Chen S, Lu B, Zhang X*. The costimulatory molecule B7-H4 promote tumor progression and cell proliferation through translocating into nucleus. Oncogene 2013, 32(46): 5347-5358.

(18)    Wang WP, Li F, Mao Y, Zhou H, Sun J, Li R, Liu CP, Chen WC, Hua D*, Zhang XG*. A miR-570 binding-site polymorphism in the B7-H1 gene is associated with the risk of gastric adenocarcinoma. Hum Genet 2013, 132(6): 641-648.

(19)    Yang P#, Tang R#, Zhu J, Zou L, Wu R, Zhou H, Mao Y, Li R, Hua D*, Wang WP*, Zhang H. A functional variant at miR-24 binding site in B7-H2 alters susceptibility to gastric cancer in a Chinese Han population.Mol Immunol2013, 56(1-2): 98-103.

(20)    Li R, Chen WC*, Pang XQ, Tian WY, Wang WP, Zhang XG. Effect of PI3K gene silencing on growth, migration and related proteins expression of CD40 signal-mediated gastric cancer cells. Mol Biol Rep 2013, 40(2): 999-1008.

(21)    Li R, Chen WC*, Pang XQ, Tian WY, Wang WP, Zhang XG. Screening and test of CD40 related signal transduction pathway in AGS cells and construction of gene silencing vector. Mol Biol Rep 2013, 40(8): 4827-35.

(22)    Mao Y, Sun J, Wang WP, Zhang XG*, Hua D*. Clinical significance of costimulatory molecule B7-H3 expression on CD3(+) T cells in colorectal carcinoma. Chinese Med J (Engl) 2013, 126(16): 3035-3038.

(23)    Li R, Chen WC*, Pang XQ, Tian WY, Wang WP, Zhang XG. Combined effect of sCD40L and PI3K siRNA on transplanted tumours growth and microenvironment in nude mice with gastric cancer. Mol Biol Rep 2012, 39(9): 8755-8761.

(24)    Li R, Chen WC*, Pang XQ, Tian WY, Wang WP, Zhang XG. CD40 signal expression in gastric cancer tissue and its correlation with prognosis of gastric cancer patients. Mol Biol Rep 2012, 39(9):8741-8747.

(25)    Wang WP#, Sun J#, Li F, Li R, Gu YP, Liu CP, Yang P, Zhu M, Chen LJ, Tian WY, Zhou H, Mao Y, Zhang L, Jiang JT, Wu CP, Hua D, Chen WC, Lu BF, Ju JF*, Zhang XG*. A frequent somatic mutation in CD274 3’-UTR leads to protein over-expression in gastric cancer by disrupting miR-570 binding. Hum Mutat 2012, 33(3): 480-484.

(26)    Li R, Chen WC*, Wang WP, Tian WY, Zhang XG. CD40 signaling activated by agonistic anti-CD40 monoclonal antibody 5C11 has different effects on biological behavior of gastric carcinoma cells. Immunol Lett2010, 131(2): 120-125.

(27)    Ren L, Gao YZ, Yin JH, Shi WX, Yu XW, Xie HP*, Wang WP*. The determination for the three genotypes of D16S539 locus based on near-infrared spectroscopy and chemical pattern recognition. Anal Chim Acta 2009, 638(2): 202-208.

(28)    Li R, Chen WC*, Wang WP, Tian WY, Zhang XG. Extraction, characterization of Astragalus polysaccharides and its immune modulating activities in rats with gastric cancer. Carbohyd Polym 2009, 78: 738-742.

(29)    Li R, Chen WC*, Wang WP, Tian WY, Zhang XG. Optimization of extraction technology of Astragalus polysaccharides by response surface methodology and its effect on CD40. Carbohyd Polym 2009, 78: 784-788.

(30)    Yin J#, Ni KY, Shen Y#, Ma PC*, Cao L, Wang WP, Wang Y*. Development and validation of a LC-MS/MS method for the determination of viaminate in human plasma. J Chromatogr B 2007, 856: 376-380.

 

k  肿瘤药物基因组学

(31)    Mao Y#, Zou CD#, Meng FY, Kong JH, Wang WP*, Hua D*. The SNPs in pre-miRNA are related to the response of capecitabine-based therapy in advanced colon cancer patients. Oncotarget 2018, 9(6): 6793-6799.

(32)    Mao Y#, Wang C#, Meng FY, Kong JH, Cao S, Jiang YG, Wang WP*, Hua D*. Polymorphisms in the ICOS/CD28-ICOSL pathway are related to capecitabine-based chemotherapy response in advanced colon cancer patients. Mol Immunol2018, 96: 78-82.

(33)    陈琦,程景洪,周芯茹,孟凡义,孔洁红,汪维鹏*,张洪建. PD-L1 基因多态性与结直肠癌患者奥沙利铂化疗疗效和安全性的相关性研究. 现代生物医学进展20181810:1828-1832.

(34)    何妙翠,王光莲,许英,汪维鹏*,华东. MiR-SNP与结直肠癌发生发展及化疗疗效关联性研究. 中华肿瘤防治杂志2018257):455-460.

(35)    Chen Q#, Mao Y#, Meng FY, Wang L, Zhang HJ, Wang WP*, Hua D*. Rs7911488 modified the efficacy of capecitabine-based therapy in colon cancer through altering miR-1307-3p and TYMS expression. Oncotarget 2017, 8(43): 94312-94319.

(36)    Chen Q#, Meng FY#, Wang L, Mao Y, Zhou H, Hua D, Zhang HJ, Wang WP*. A polymorphism in ABCC4 is related to efficacy of 5-FU/capecitabine-based chemotherapy in colorectal cancer patients. Sci Rep 2017, 7(1): 7059.

(37)    Zhu JJ, Chen LX, Mao Y, Zhou H, Li R*, Wang WP*. Multiplex allele-specific amplification from whole blood for detecting multiple polymorphisms simultaneously. Genet Test Mol Bioma2013, 17(1): 10-15.

(38)    Cao P, Wang QJ, Zhu XT, Zhou H*, Li R, Wang WP*. Quantitative determination of allele frequency in pooled DNA by using sequencing method. J Chromatogr B2011, 879(7-8): 527-532.

(39)    Wang WP*, Zhang XD, Zhou GH*. High-throughput genotyping by coupling adapter-ligation mediated allele-specific amplification with microplate array parallel gel electrophoresis. Mol Biotechnol 2010, 44(1): 1-7.

(40)    Wang WP*, Zhang RH, Wu P, Wang S, Li R. Estimation of allele frequency in pooled DNA by using PCR-RFLP combined with microchip electrophoresis.J Chromatogr B 2009, 877(14-15): 1603-1606.

(41)    Ren L, Wang WP, Gao YZ, Yu XW, Xie HP*. Typing SNP based on the near-infrared spectroscopy and artificial neural network. Spectrochim Acta A 2009, 73(1): 106-111.

(42)    汪维鹏*,吴萍,王森,李融. 基于近红外光谱-支持向量机技术识别单碱基差异.苏州大学学报医学版2009294):652-655.

(43)    汪维鹏*,李融,王森. 单管等位基因特异性扩增法同时测定多重单核苷酸多态性.南京医科大学学报自然科学版2009284):556-561.

(44)    汪维鹏,周国华*.多重等位基因特异性扩增-微流控芯片电泳法同时测定多个单核苷酸多态性位点. 遗传2009312):219-224.

(45)    Wang WP, Sun WM, Wu WJ, Zhou GH*. Improved adapter-ligation mediated allele-specific amplification for multiplex genotyping by using software. Electrophoresis 2008, 29(7): 1490-1501.

(46)    Wang WP, Wu HP, Zhou GH*. Detection of avian influenza a virus by using Pyrosequencing. Chinese J Anal Chem 2008, 36(6): 775-780.

(47)    Wang WP, Ni KY, Zhou GH*. Association of IL1B polymorphisms with gastric cancer in a Chinese population. Clin Biochem 2007, 40(3-4):218-25.

(48)    Zhang J, Zhu FY, Pu YP*, Yin LH, Luo J, Wang WP, Zhou GH. Analysis of Multiple Single Nucleotide Polymorphisms (SNPs) of Myeloperoxidase (MPO) to Screen for Genetic Markers Associated with Acute Leukemia in Chinese Han Population. J Toxicol Environ Health A 2007, 70(11): 901-907.

(49)    Wang WP, Ni KY, Zhou GH*. Multiplex SNP genotyping by adapter-ligation mediated allele-specific amplification. Anal Biochem 2006, 355(2): 240-248.

(50)    Wang WP, Ni KY, Zhou GH*. Microchip electrophoresis coupled with multiplex polymerase chain reaction for typing multiple single nucleotide polymorphisms simultaneously. Chinese J Anal Chem 2006, 34(10): 1389-1394.

(51)    汪维鹏,倪坤仪,周国华*. 连接反应介导的等位基因特异性扩增-微流控芯片电泳法同时检测多个SNP位点. 高等学校化学学报20062710):1856-1858.

(52)    汪维鹏,倪坤仪,周国华*. 一种基于适配器连接介导的等位基因特异性扩增法测定多重SNP. 遗传2006282):219-226.

(53)    汪维鹏,倪坤仪,周国华*. 单核苷酸多态性检测方法的研究进展. 遗传2006281):117-126.

 

k  肿瘤药物代谢动力学

(54)    Kong JH#, Qiu YJ#, Li Y, Zhang HJ*, Wang WP*. TGF-β1 elevates P-gp and BCRP in hepatocellular carcinoma through HOTAIR/miR-145 axis. Biopharm Drug Dispos 2019, 40(2):70-80.

(55)    黄洁琼,徐云婷,李佳俊,汪维鹏* ,张洪建*. 构建MDCK II-hMD1-hCYP3A4 共表达体系. 药物生物技术. 201724( 4) : 289-293.

(56)    李远,黄洁琼,李佳俊,汪维鹏*,张洪建*. CYP2C8 CYP3A4 细胞表达体系的构建及其在小分子激酶药物对紫衫醇代谢途径抑制研究中的应用. 生物技术通报201632(7)1-6.

(57)    Chen QX, Wang WP, Zeng S, Urayama S, Yu AM*. A general approach to high-yield biosynthesis of chimeric RNAs bearing various types of functional small RNAs for broad applications. Nucleic Acids Res 2015, 43(7):3857-3869.

(58)    Li MM, Addepalli B, Tu MJ, Chen QX, Wang WP, Limbach PA, LaSalle J, Zeng S, Huang M, Yu AM*. Chimeric miR-1291 biosynthesized efficiently in E. coli is effective to reduce target gene expression in human carcinoma cells and improve chemosensitivity. Drug Metab Dispos 2015, 43(7):1129-1136.

(59)    Li MM, Wang WP, Wu WJ, Huang M, Yu AM*. Rapid production of novel pre-microRNA agent hsa-mir-27b in Escherichia coli using recombinant RNA technology for functional studies in mammalian cells. Drug Metab Dispos 2014, 42(11): 1791-1795.

(60)    王玲娜,吴海瑛,陈临琪,汪维鹏*.  高效液相色谱-四级杆飞行时间串联质谱的儿童性早熟代谢组学研究. 中国临床药理学杂志20143011):1002-1005.

(61)    He G, Qi H, Wang M, Yang J, Wen F, Wang W, Qiao C, Zhang H*. LC-MS/MS method for the simultaneous quantitation of three active components derived from a novel prodrug against schistosome infection. J Pharmaceut Biomed 2013, 83:186-193.

(62)    王亚,汪维鹏*,张洪建. 液相色谱-串联质谱法测定猴血浆对乙酰氨基酚浓度及其药代动力学研究. 现代医药卫生20132917):26-29.

(63)    Wu H, Wu W, Chen Z, Wang W, Zhou G*, Kajiyama T, Kambara H. Highly sensitive pyrosequencing based on the capture of free adenosine 5' phosphosulfate with adenosine triphosphate sulfurylase. Anal Chem 2011, 83(9):3600-5.

(64)    汪维鹏,古卓良,倪坤仪,余书勤,周国华. HPLC法测定犬血浆中盐酸伪麻黄碱浓度及西嗪伪麻缓释片的初步体内药动学研究. 中国药学杂志2005407):535-537.

(65)    汪维鹏,丁黎. 柱切换HPLC技术及其在体内药物分析中的应用. 药学进展2003275):274-278.



2、项目

12019-2022:江苏省重点研发计划(社会发展)项目(BE2019657),新型抗肿瘤靶标SRSF3的鉴定及其小分子抑制剂的开发,主持。

22018-2021:国家自然科学基金面上项目 (81773044)结直肠癌中B7-H3亚型异常表达的调控机制及功能意义研究,主持。

32015-2018:国家自然科学基金面上项目 (81473278)合成生物学在阐明药物代谢酶及转运体相互关系研究中的应用,第2

42014-2017:国家自然科学基金面上项目 (81372375)miR-SNP介导卡培他滨结直肠癌化疗反应个体差异的分子机制研究,第2

52013-2016:国家自然科学基金青年-面上连续项目 (81270031)负性协同刺激分子B7-H1 基因变异在胃癌发生发展过程中的作用机制研究,主持。

62010-2012:国家自然科学基金青年项目 (30901360),负性协同刺激分子B7-H1在胃癌组织中表达的调节机制研究,主持。


3、获奖

12019:江苏高校“青蓝工程”优秀青年骨干教师培养对象

22018:教育部高等学校科学研究优秀成果奖(科学技术)二等奖,第2

32018:江苏省科技进步二等奖,第2

42017:华夏医学科技奖一等奖,第5

52017:江苏医学科技奖一等奖,第3

62017:江苏省医学新技术引进二等奖,第2

72012:中国免疫学会青年学者奖


4、专利

1Yu AM, Wang WP, Chen QX, Li MM. HYBRID tRNA/pre-miRNA MOLECULES AND METHODS OF USE. PCT/US10619156B2.

2汪维鹏、孟凡义、邱雅静、孔洁红、陈喜华. 微小RNA及其在制备抗肿瘤药物中的应用,授权号:ZL201711189410.5PCT/CN2018/077481

(3汪维鹏、王蕾、陈琦. ABCC4基因多态性位点rs3742106的用途及其检测引物与试剂盒,授权号:ZL201510717958.7


5、著作 

1)《药物分析(案例版)》(参编),科学出版社,2017。 

2)Weipeng Wang, Haiping Wu, Bingjie Zou, Qinxin Song, Guohua Zhou. Detection of Avian Influenza A Virus by Pyrosequencing. Advances and Clinical Practice in Pyrosequencing, 2016, 371-380. 

3)《SNP检测技术与个体化药物治疗》(副主编),苏州大学出版社,2015 

4《药物分析实验教程》(副主编),合肥工业大学出版社出版,2009。 

5)《创新药物化学(译著)》(参编),世界图书出版社,2005