苏州大学药学院欢迎您!

杨霜

发布者:金雪明发布时间:2020-10-16浏览次数:1867


本实验室诚聘糖生物、蛋白质组学、糖组学、质谱分析和蛋白生物信息学副教授、讲师和博士后。


任职


特聘教授

苏州大学药学院药物分析系临床质谱中心


联系方式


苏州市工业园区仁爱路199号苏州大学独墅湖校区二期云轩楼2107

电话:(05216588 0229 (办公室); 134 0506 4922 (手机)

邮箱:yangs2020@suda.edu.cn


Current Appointments

            2020/10 ~

            Professor

            Director, Center for Clinical Mass Spectrometry

            School of Pharmaceutical Sciences

            Soochow University

 

Contact Information

            2107 Yuxuan Bldg, 199 Renai Rd

            Dushu Lake, Industrial Park

            Jiangsu, China

            Tel: (1) 410-302-4681; (86) 134-0506-4922

            Email: yangs2020@suda.edu.cn

 

Education and Training (in chronological order):

Undergraduate:      1990/9-1994/7               

                              B.S., Chemistry, Harbin Institute of Technology, Heilongjiang, China  

Master/graduate:     1994/9-1997/3               

                              M.S., Materials Science and Engineering, Shanghai Jiaotong University, Shanghai, China

Master/graduate:     2002/1-2003/7               

                              M.S., Materials Science, National University of Singapore, Singapore

Doctor/graduate:     2003/8-2008/12             

                              Ph.D., Mechanical Engineering, University of Maryland, College Park 

Scientist/Postdoc:   2009/1-2010/12             

                              Postdoc, IO Scientific & University of Maryland, MML, College Park, MD   

Postdoc:                2011/1-2013/9

                              Postdoc, Johns Hopkins University, Pathology, MD

 

Professional Experience (in chronological order):               

 

1994/9-1997/3          Research Assistant, Shanghai Jiaotong University, Shanghai, China

1997/3-1999/12        Quality Control Manager, Shanghai Hawei New Materials & Technology Co., Ltd, Shanghai, China

2000/1-2001/12        Technical Support Engineer, Samsung Cheil Industries Inc., Shanghai, China

2002/1-2003/7          Research Assistant, National University of Singapore, Singapore

2003/8-2008/12        Research/Teaching Assistant, Pre-doctoral Trainee, University of Maryland, College Park, MD      

2009/1-2010/12      Research Scientist, IO Scientific; Postdoc Training, University of Maryland, MD

2011/1-2013/9          Research Fellow, Department of Pathology, Johns Hopkins University, Baltimore, MD    

2013/10-2016/10      Junior Faculty, Department of Pathology, Johns Hopkins University, Baltimore, MD

2016/10-2019/09      Staff Fellow, Laboratory of Bacterial Polysaccharides, CBER, FDA, Silver Spring, MD

2019/10-2020/09      Staff Scientist, Biological Chemistry Section, NIDCR/NIH, Bethesda, MD

                                         

RESEARCH ACTIVITIES

Awards, honors

2008                       Best Poster Award, HPLC 2008 for Innovation in Pharmaceutical Analysis, Baltimore, MD

2011                       Travel Award, 2011 Glycobiology Society Annual Meeting, Seattle, WA

2012                       Young Investigator Award, 2012 14th Annual Department of Pathology Research Day, Baltimore, MD

2012                       Travel Award, The 11th World Conference of 2012 HUPO, Boston, MA

2012                       Presentation Award, 3rd place. The 11th World Conference of 2012 HUPO, Boston, MA

2013                       Best Poster Award. 2013NIH/FDA Glycosciences Research Day, Bethesda, MD

2013                       Travel Award, 2013 22nd International Symposium on Glycoconjugates, Dalian, China

2017                       Young Investigator Award, 2017 ACS Pittcon, CACA, Chicago, IL

2018                       FDA CBER Scientific Achievement Award, US FDA, Silver Spring, MD

 

Educational Publications

Book chapters

1.      S. Yang and D. DeVoe, Chapter “Microfluidic device fabrication by thermoplastic hot-embossing,” Edited by G. Jenkins, C.D. Mansfield, “Microfluidic Diagnostics” in “Methods in Molecular Biology,” Humana Press, Springer, 2012.

2.      S. Yang* and K.Q. Jiang, Chapter “Elastomer Application in Microsystem and Microfluidics,” in “Elastomer” 2012, InTech, 203-222.

3.      S. Li, S.N. Thomas, H. Zhang, and S. Yang*, Chapter “Glycoprotein Biomarkers” in “Targeted Biomarker Quantitation by LC-MS (Wiley)”, 2017, 246-272.

4.      S. Yang*, Chapter “Glycoprotein and Glycosylation Bioinformatics” (Chinese), 2021.

 

Patents

02/05/2021        S. Yang, S. Li, Preparation of solid-phase isobaric tag and for peptide/glycopeptide quantification. Soochow University. Application No. 202110163100.6.

01/08/2021        S. Yang, A. Yang, A medical device for separation of biofluid from tissue specimens. Soochow University. Application No. 202120042939.X.

12/16/2020        S. Yang, S. Li, Solid-phase enrichment of N- and O-linked glycopeptides, Soochow University. Application No. 202011481041.9.

07/27/2018        S. Li, M. Wang, S. Yang, Isobaric tags for quantification of oligosaccharide, Nanjing Apollomics. Application No. 20180843616.3.

06/13/2017        S. Yang, H. Zhang, AutoTip for high-throughput analysis of N-glycans, Johns Hopkins University, pending.

10/12/2015        S. Li, S. Yang, and H. Zhang, Quaternary amine containing isobaric tag for quantitative glycan profiling. University of Maryland and Johns Hopkins University. US patent 20160252518 A1 (US 10,254,291 B2)

05/13/2014        H. Zhang, S. Yang, P. Shah, S. Sun, Solid phase glycan and glycopeptide analysis and microfluidic chip for glycomic extraction, analysis and methods for using same. Johns Hopkins University. World patent #2,014,040,066 A1; US 2015/0233903 A1.

03/13/2014        H. Zhang, S. Yang, Shuwei Li, Isobaric aldehyde-reactive tags for and analysis of glycans using same. Johns Hopkins University. World patent #2,014,040,072 A1; US 2015/0241437 A1 (US 99,394,44 B2).

12/13/2012        H. Zhang, S. Yang. Glycan and glycopeptide capture and release using reversible hydrazone-based method. Johns Hopkins University. Patent #WO2012170491A1; US20 12/04 1050.

Publications

https://scholar.google.com/citations?hl=en&user=sQmBEbsAAAAJ&view_op=list_works&sortby=pubdate

(*corresponding author)

1.     Q. Wang, T. Wang, S. Yang, C-y. Chung, C-t. Li, J.F. Cipollo, M.J. Betenbaugh*, “The interplay of protein engineering and glyco-engineering to generate better-refined antibodies in Chinese hamster ovary cells,” in submission, 2021.

2.     S. Yang*, Y. Wang, Q. Wang, and J.F. Cipollo, “Express cells impact glycosite occupancy and types of glycans on SARS-CoV-2 spike glycoprotein,” submitted to ACS Infectious Diseases, 2021.

3.     U.H. Mortensen*, D.C. Anyaogu, A.H. Hansen, J.B. Hoof, N.I. Majewska, J.T. Paul, K.R. Nielsen, T.J. Hobley, S. Yang, H. Zhang, and M. Betenbaugh, “Glycoengineering of aspergillus nidulans to produce precursors for humanized N-glycan structures,” Metabolic Engineering 2021, revision. (IF: 7.263)

4.     M. Xu, W. Hu, Z. Liu, J. Xia, S. Chen, P.G. Wang, S. Yang*, “Glycoproteomic bioanalysis of exosomes by LC-MS to early diagnose pancreatic cancer,” Bioanalysis 2021, under review. (IF: 2.371)

5.     S. Yang*, J. Xia, Z. Yang, M. Xu, S. Li, “Lung cancer molecular mutations and abnormal glycosylation as biomarkers for early diagnosis”, Cancer Treatment and Research Communications 2021, 21(7), 100311, 1-11 (IF: 0.580)

6.     M. Xu, M. Zhou, S. Li, X. Zhen, and S. Yang*, “Glycoproteins as diagnostic and prognostic biomarkers for neurodegenerative diseases: a glycoproteomic approach,” Journal of Neuroscience Research 2021, 00, 1-17. (IF: 3.921)

7.     H. Yu, J. Wang, Z. Tang, X. Li, M. Yin, F. Zhang, J. Shu, W. Chen, S. Yang*, and Z. Li*, “Integrated glycomics strategy for evaluation of glycosylation alterations in salivary proteins associated with type 2 diabetes mellitus,” RSC Advances 2020, 10, 39739-39752. (IF: 3.267)

8.     S. Yang, Y. Wang, M. Mann, Q. Wang, E. Tian, L. Zhang, J.F. Cipollo, K.G.T. Hagen, and L.A. Tabak*, “Improved online LC-MS/MS identification of O-glycosites by EThcD fragmentation, chemoenzymatic reaction, and SPE enrichment,” Glycoconjugate Journal 2020, doi: 10.1007/s10719-020-09952-w. (IF: 3.010)

9.     S. Yang*, W. Wu, R-f Shen, L. Parsons, and J.F. Cipollo*, “Optimization of O-GIG for O-Glycopeptide Characterization with Sialic Acid Linkage Determination,” Analytical Chemistry 2020, 92, 10946-10951. (IF: 6.785)

10.  Q. Wang, T. Wang, S. Yang, S. Sha, W.W. Wu, Y. Chen, J.T. Paul, R-f. Shen, J.F. Cipollo, and M.J. Betenbaugh*, “Metabolic Engineering Challenges to extending N-glycan pathways in Chinese hamster ovary cells,” Metabolic Engineering, 2020, 61, 301-314. (IF: 7.263)

11.  N. de Haan*, S. Yang, J.F. Cipollo, and M. Wuhrer, “Glycomics studies using sialic acid derivatization and mass spectrometry,” Nature Reviews Chemistry 2020, 4, 229-242. (IF: 33.566)

12.  M. Lorna A. De Leoz et al., “NIST interlaboratory study on glycosylation analysis of monoclonal antibodies: comparison of results from diverse analytical methods,” Molecular & Cellular Proteomics 2020, 19(1), 11-30.  (IF: 4.870)

13.  C. Chung, Q. Wang, S. Yang, S. Chough, J.F. Cipollo, J.P. Balthasa, and M.J. Betenbaugh*, “The impact of sialylation linkage-type on the pharmacokinetics of recombinant butyrylcholinesterases,” Biotechnology and Bioengineering 2019, 117(1), 157-166. (IF: 4.260)

14.  H. Wan*, J. Gao, H. Yang, S. Yang, R. Harvey et al., “The neuraminidase of A(H3N2) influenza viruses circulating since 2016 is antigenically distinct from the A/Hong Kong/4801/2014 vaccine strain,” Nature Microbiology 2019, 4, 2216-2225. (IF: 14.300)

15.  S. Yang, Z. Yang, P. Wang*, “Bioanalysis for precision medicine”, Bioanalysis 2019, 11(11), 1039-1043. (IF: 2.371)

16.  S. Yang*, P. Onigman, W. Wu, J. Sjogren, H. Nyhlen, R-F. Shen, and J. Cipollo*, “Deciphering protein O-glycosylation: solid-phase chemoenzymatic cleavage and enrichment,” Analytical Chemistry 2018, 90(13), 8261-8269 (ACS Editor’s Choice). (IF: 6.785)

17.  S. Yang*, W. Wu, R-F. Shen, M. Bern, and J. Cipollo*, “Identification of sialic acid linkages on intact glycopeptides via differential chemical modification using intactGIG-HILIC,” Journal of the American Society for Mass Spectrometry 2018, 29(6), 1273-1283. (IF: 3.150)

18.  S. Yang*, S. Chatterjee*, and J. Cipollo, “The glycoproteomics-mass spectrometry for studying cardiac hypertrophy and heart failure,” Proteomics-Clinical Applications 2018, 12(5), 1870042, 1-13. (IF: 2.568)

19.  N. Hoti, S. Yang, Y. Hu, P. Shah, M. Haffner, and H. Zhang*, “Overexpression of α (1,6) fucosyltransferase in the development of castration-resistant prostate cancer cells,” Prostate Cancer and Prostatic Diseases 2018, 21(1), 137, 1-10. (IF: 4.311)

20.  W. Zhou, S. Yang, P.G. Wang*, “Matrix effects and matrix effect factors,” Bioanalysis 2017, 9(23), 1839-1844. (IF: 2.371)

21.  S. Yang*, D. Clark, Y. Liu, S. Li, H. Zhang, “High-throughput analysis of N-glycans using AutoTip via glycoprotein immobilization for glycan extract,” Scientific Reports 2017,7(1), 10216, 1-11. (IF: 3.998)

22.  S. Yang*, L. Chen, D.W. Chen, Q. Li, and H. Zhang, “Protein signatures of molecular pathways in non-small cell lung carcinoma (NSCLC): comparison of glycoproteomics and global proteomics,” Clinical Proteomics 2017,14(1), 31, 1-15. (IF: 2.568)

23.  S. Yang, E. Jankowska, M. Kosikova, H. Xie, and J. Cipollo*, “Solid-Phase Chemical Modification for Sialic Acid Linkage Analysis: Application to Glycoproteins of Host Cells Used in Influenza Virus Propagation,” Analytical Chemistry 2017, 89(17), 9508-9517. (IF: 6.785)

24.  N. Hoti*, S. Yang, P. Aiyetan, B. Kumar, Y. Hu, D. Clark, A.U. Eroglu, P. Shah, T. Johnson, W.H. Chowdery, H. Zhang, and R. Rodriguez, “Overexpression of Exportin-5 Overrides the Inhibitory Effect of miRNAs Regulation Control and Stabilize Proteins via Posttranslation Modifications in Prostate Cancer,” Neoplasia 2017, 19(10), 817-829. (IF: 5.696)

25.  S. Yang* and P. Wang, “Method development of glycoprotein biomarkers for cancers,” Bioanalysis 2017; 9(12):903-906. (IF: 2.371)

26.  C. Chung, Q. Wang, S. Yang, S.A. Ponce, B.J. Kirsch, H. Hang, M.J. Betenbaugh*, “Combinatorial genome and protein engineering yields monoclonal antibodies with hypergalactosylation from CHO cells,” Biotechnology and Bioengineering 2017, 114(12), 2848-2856. (IF: 4.002)

27.  S. Yang*, L. Zhang, S. Thomas, Y. Hu, S. Li, J. Cipollo, and H. Zhang, “Modification of sialic acids on solid-phase: accurate characterization of protein sialylation,” Analytical Chemistry 2017, 89(12), 6330-6335. (IF: 6.785)

28.  S. Yang*, Y. Hu, L. Sokoll, and H. Zhang*, “Simultaneous quantification of N- and O-glycans using a solid-phase method”, Nature Protocols 2017, 12(6), 1229-1244. (IF: 10.419)

29.  S. Yang*, N. Hoti, W. Yang, Y. Liu, S. Li, and H. Zhang*, “Simultaneous analyses of N-linked and O-linked glycans using solid-phase chemoenzymatic method,” Clinical Proteomics 2016, 14(3), 1-11. (IF: 2.568)

30.  C. Chung, Q. Wang, S. Yang, B. Yin, H. Zhang, M. Betenbaugh*, “Integrated Genome and Protein Editing Swaps α2, 6 Sialylation for α2, 3 Sialic Acid on Recombinant Antibodies from CHO,” Biotechnology Journal 2017, 12(2), 1600502, 1-11. (IF: 3.910)

31.  N. Hoti*, P. Shah, Y. Hu, S. Yang, and H. Zhang, “Proteomics analyses of prostate cancer cells reveals cellular pathways associated with androgen resistance,” Proteomics 2017, 17, 6, 1-10. (IF: 3.254)

32.  D. Do, S. Yang, X. Yao, R.G. Hamilton, J.T. Schroeder, P. Gao*, “N-glycan in cockroach allergen regulates human basophil function,” Immunity, Inflammation and Disease 2017, 5(4), 386-399. (IF: 2.625)

33.  X. Jia, J. Chen, S. Sun, W. Yang, S. Yang, P. Shah, N. Hoti, B. Veltri, and H. Zhang*, “Detection of Aggressive Prostate Cancer-Associated Glycoproteins in Urine using Glycoproteomics and Mass Spectrometry,” Proteomics 2016, 16(23), 2928. (IF: 3.254)

34.  S. Yang, M. Wang, L. Chen, B. Yin, G. Song, I.V. Turko, K.W. Phinney, M.J. Betenbaugh, H. Zhang*, and S. Li*, “QUANTITY: an isobaric tag for quantitative glycomics,” Scientific Reports, 2015, 5,17585, 1-10. (IF: 3.998)

35.   P. Shah, X. Wag, W. Yang, S.Toghi Eshghi, S. Sun, N. Hoti, L. Chen, S. Yang, J. Pasay, A. Rubin, and H. Zhang*, “Integrated proteomic and glycoproteomic analyses of prostate cancer cells reveals glycoprotein alteration in protein abundance and glycosylation,” Molecular & Cellular Proteomics 2015, 14(10), 2753-2763. (IF: 4.870)

36.  B. Yin, Y. Gao, C. Chung, S. Yang, E. Blake, M.C. Stuczynski, J. Tang, H.F. Kildegaard, M.R. Anderson, H. Zhang, and M.J. Betenbaugh*, “Glycoengineering of Chinese hamster ovary cells for enhanced erythropoietin N-glycan branching and sialylation,” Biotechnology and Bioengineering 2015, 112 (11), 2343-2350. (IF: 4.002)

37.  S. Yang*, A. Rubin, S. Toghi Eshghi, and H. Zhang, “Chemoenzymatic method for glycomics: Isolation, identification, and quantitation,” Proteomics 2016, 16, 241-256. (IF: 3.254)

38.  S. Yang*, S. Mishra, L. Chen, J. Zhou, D.W. Chan, S. Chatterjee, and H. Zhang, “An integrated glycoprotein immobilization method for glycopeptide and glycan analysis of cardiac hypertrophy,” Analytical Chemistry 2015, 87(19), 9671-9678. (IF: 6.785)

39.  S. Sun, P. Shah, S. Toghi Eshghi, W. Yang, N. Trikannad, S. Yang, L. Chen, P. Aiyetan, N. Hoti, Z. Zhang, D.W. Chan, and H. Zhang*, “Solid-phase extraction of N-linked glycans and glycosite-containing peptides (NGAG) for comprehensive characterization of glycoproteins,” Nature Biotechnology 2016, 34(1), 84-88. (IF: 36.553)

40.  P. Shah, B. Zhang, C. Choi, S. Yang, J. Zhou, R. Harlan, Y. Tian, Z. Zhang, D.W. Chan, and H. Zhang*, “Tissue proteomics using chemical immobilization and mass spectrometry,” Analytical Biochemistry 2015, 469, 27-33. (IF: 2.877)

41.  S. Yang*, L. Chen, P. Shah, W. Yang, B. Zhang, Z. Zhang, D.W. Chan, D.A. Kass, J.E. Eyk, and H. Zhang, “Glycoproteins identified from heart failure and treatment models,” Proteomics 2015, 15(0), 567-579. (IF: 3.254)

42.  S. Toghi Eshghi, S. Yang, X. Wang, P. Shah, X. Li, and H. Zhang*, “Imaging of N-linked glycans from formalin-fixed paraffin-embedded tissue sections using MALDI mass spectrometry,” ACS Chemical Biology 2014, 9(9), 2149-2156. (IF: 4.434)

43.  W. Yang, P. Shah, S. Toghi Eshghi, S. Yang, S. Sun, M. Ao, A. Rubin, J. Jackson, and H. Zhang*, “Glycoform-analysis of recombinant and human deficiency virus envelope protein gp120 via higher energy C-trap dissociation and spectral-aligning strategy,” Analytical Chemistry 2014, 86(14), 6959-6967. (IF: 6.785)

44.  S. Yang* and H. Zhang, “Glycomic analysis of glycans released from glycoproteins using chemical immobilization and mass spectrometry,” Current Protocols in Chemical Biology 2014, 6, 191-208.

45.  S. Yang, S. Toghi Eshghi, H. Chiu, D.L. DeVoe, and H. Zhang*, “Glycomic analysis by glycoprotein immobilization for glycan extraction and liquid chromatography on microfluidic chip,” Analytical Chemistry 2013, 85(21), 10117-10125. (IF: 6.785)

46.  S. Yang, W. Yuan, W. Yang, J. Zhou, R. Harlan, J.L. Edwards, S. Li, and H. Zhang*, “Glycan analysis by isobaric aldehyde reactive tags and mass spectrometry,” Analytical Chemistry 2013, 85(17), 8188-8195. (IF: 6.785)

47.  S. Yang, Y. Li, P.K. Shah, and H. Zhang*, “Glycomic analysis using glycoprotein immobilization for glycan extraction,” Analytical Chemistry 2013, 85(11), 5555-5561. (IF: 6.785)

48.  P.K. Shah, S. Yang, S. Sun, P. Aiyata, K.J. Yarema, and H. Zhang*, “Mass spectrometric analysis of sialylated glycans using solid phase labeling of sialic acids,” Analytical Chemistry 2013, 85(7), 3606-3613. (IF: 6.785)

49.  S. Yang, and H. Zhang*, “Solid-phase glycan isolation for glycomics analysis,” Proteomics: Clinical Applications 2012, 6(11-12), 596-608. (IF: 2.568)

50.  P. Hareesh, I. Misri, S. Yang, D.L. DeVoe, “Transverse integrated electrode actuation of homogenous bulk PZT,” IEEE/ASME Journal of Microelectromechanical Systems 2012, 21(6), 1513-1518. (IF: 5.673)

51.  I. Misri, P. Hareesh, S. Yang, D.L. DeVoe, “Microfabrication of bulk PZT transducers by dry film photolithography and micro powder blasting,” Journal of Micromechanics and Microengineering 2012, 22, 085017, 1-10. (IF: 1.739)

52.  S. Yang and H. Zhang*, “Glycan analysis by reversible reaction to hydrazide beads and mass spectrometry,” Analytical Chemistry 2012, 84, 2232-2238. (IF: 6.785)

53.  J. Liu, J. Chen, S. Yang, C.C. Zhang, and D. DeVoe*, “Mixed-mode electrokinetic and chromatographic peptide separations in a microvalve-integrated polymer chip,” Lab on a Chip 2010, 10, 2122-2129. (IF: 6.774)

54.  S. Yang*, J. Wu, and M. Pecht, “Reliability assessment of land grid array sockets subjected to mixed flowing gas environment,” IEEE Transactions on Reliability 2009, 58(4), 634-640. (IF: 3.117)

55.  S. Yang and D. DeVoe*, “Microfluidic two-dimensional IEF-CZE for proteomics,” Proceedings of International Solid-State Sensors, Actuators and Microsystems Conference, Transducers 2009, Denver, CO, Jun 21-25, 2009.

56.  S. Yang, J. Liu, C.S. Lee, and D. DeVoe*, “Microfluidic 2-D PAGE using multifunctional in-situ polyacrylamide gels and discontinuous buffers,” Lab on a Chip 2009, 9, 592-599. (IF: 6.774)

57.  S. Yang, J. Liu, and D. DeVoe*, “Spatially-multiplexed protein separations enabled by multifunctional photopolymerized gels,” Proceedings of the 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2008), San Diego, CA, Oct 12-16, 173-175.

58.  J. Liu, S. Yang, C.S. Lee, and D. DeVoe*, “Polyacrylamide gel plugs enabling 2-D microfluidic protein separations via IEF and multiplexed SDS gel electrophoresis,” Electrophoresis 2008, 29(11), 2241-2250. (IF: 3.081)

59.  S. Yang, J. Liu, and D. DeVoe*, “Optimization of sample transfer in two-dimensional microfluidic separation systems,” Lab on a Chip 2008, 8, 1145-1152. (IF: 6.774)

60.  S. Yang*, and A. Christou*, “Failure model for silver electrochemical migration,” IEEE Transactions on Device and Materials Reliability 2007, 7 (1), 188-196. (IF: 1.790)

61.  S. Yang, J. Wu, D. Tsai, and M. Pecht*, “Contact resistance estimation for time-dependent silicone elastomer matrix of land grid array socket,” IEEE Transactions on Components and Packaging Technologies 2007, 30(1), 81-85. (IF: 1.889)

62.  S. Yang, J. Wu, and A. Christou*, “Initial stage of silver electrochemical migration degradation,” Microelectronics Reliability 2006, 46,1915-1921. (IF: 1.535)

63.  J.S. Buch, J. Liu, S. Yang, C.-W. Tsao, T. Song, P. Sivanesan, K. Phalnikar1, C.S. Lee, and D.L. DeVoe*, “Automated two-dimensional microfluidic protein profiling combining IEF and SDS gel electrophoresis,” Proceedings of 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2006), Tokyo, Japan, Nov 5-9, 1579-1581.

64.  S. Yang, J. Wu, and M. Pecht*, “Electrochemical migration of land grid array sockets under highly accelerated stress conditions,” Proceedings of 51st IEEE Holm Conference, Chicago, IL, Sep 26-29, 2005, 238-244.

65.  S. Yang, Y-W Zhang, and K. Zeng*, “Analysis of nanoindentation creep for polymeric materials,” Journal of Applied Physics 2004, 95 (7), 3655-3666. (IF: 2.286)

66.  S. Yang, K. Sun, R. Wu*, “Research on damping property of hybrid fiber composites,” Fiber Composites 2002, 19(1), 6 (Chinese). (IF: 0.464)

67.  S. Yang, K. Sun, R. Wu*, “Research on E-51 epoxy curing system,” Fiber Reinforced Plastics/Composites 2002, 2, 32 (Chinese). (IF: 1.503)

68.  S. Yang, K. Sun, R. Wu*, “Relationships between parameter design and mechanical properties of hybrid composites,” Fiber Composites 2001, 18(4), 14 (Chinese). (IF: 0.464)

 

Papers in preparation/review

1.     W. Hu, M. Xu, J. Zhang, S. Yang, “Abnormal glycosylation in metastatic lung adenocarcinomas,” in preparation.

2.     Q. Wang, S. Yang, et al., “Comprehensive glycoproteomic analysis of multiple Chinese hamster ovary host cell lines,” in preparation.

3.     Q. Wang, S. Yang et al., “The interplay of protein engineering and glyco-engineering to fine-tune antibody glycosylation with better effector functions,” in preparation.

4.     J. Zhang, Z.L. Liu, C.S. Gui, P.G. Wang, S. Yang, “Advanced analysis of protein glycosylation by HILIC-MS,” in preparation (review article).

5.     W. Hu, M. Xu, Z.L. Liu, J. Xia, X. Zhang, and S. Yang, “Regulated glycosylation in lung adenocarcinomas,” in preparation.

6.     Q. Wang, S. Yang, T. Wang, L. Parsons, W. Wu, M.J. Betenbaugh, J.F. Cipollo, “Advanced N- and O-glycosylation characterization of multiple Chinese hamster ovary host cell lines using HILIC-GIG method,” in preparation.

7.     S. Yang, W.Wu, R-F Shen, and J.F. Cipollo, “A microscale O-glycan isolation by β-elimination and permethylation,” in preparation.

8.     S. Yang, F. Assis, W.Wu, J. Sjogren, L. Parsons, H. Nyhlen, P. Onigman, R-f Shen, M. Rios, and J.F. Cipollo, “Glycosylation migration in Zika virus strains from 1947 to 2016 using a novel lectin solid-phase enrichment,” in preparation.

9.     S. Yang, H. Wan, M. Eichelberger, Z. Ye, and J. Cipollo, “Interaction of different influenza virus neuraminidases on removal of specific sialic acids from sialyl-glycoproteins,” in preparation.

10.  C. Chuang, Q. Wang, S. Yang, J. Cipollo, M. Betenbaugh, “Glycoengineering on IgG antibody,” preparing for Biotechnology and Bioengineering.

11.  S. Yang, W. Wu, M. Kosikova, X. Hang, R-f. Shen, and J. Cipollo, “Sialic linkage migration in cell passage: an indication to cell virus infectivity,” preparing for Journal of Virology.

 

Oral Presentation or Posters

1.     2020/6/3, Metabolic engineering challenges to extending N-glycan pathways in CHO cells, 68th ASMS ASMS Conference on Mass Spectrometry and Allied Topics, Houston, TX.

2.     2020/6/2, Comprehensive N- and O-glycosylation characterization of multiple CHO host cell lines using HILIC-GIG method, 68th ASMS ASMS Conference on Mass Spectrometry and Allied Topics, Houston, TX.

3.     2019/7/8, GlycoCatch lectin for comprehensive analysis of protein glycosylation, 2019 NIH&FDA Glycoscience Research Day, Bethesda, MD.

4.     2019/6/2-6, Analysis of Zika Viral Polyprotein N- and O-glycosylation Using a Novel Lectin-chemoenzymatic Enrichment, 67th ASMS Conference on Mass Spectrometry and Allied Topics, Atlanta, GA.

5.     2018/8/19-23, Host ANAL DIVISION session on “solid-phase chemoenzymatic for glycosylation analysis”, 256th ACS Fall National Meeting, Boston, MA. (oral)

6.     2018/7/13, Solid-phase chemoenzymatic method for cleavage and enrichment of protein O-glycosylation, NIH/FDA Glycoscience Research Day, Bethesda, MD. (oral)

7.     2018/6/3-7, Deciphering complex O-glycosylation: solid-phase chemoenzymatic cleavage and enrichment, 66th ASMS Conference on Mass Spectrometry and Allied Topics, San Diego, CA.

8.     2018/6/3-7, “A mass spectrometry based glycomics platform for analysis of influenza vaccines,” 66th ASMS Conference on Mass Spectrometry and Allied Topics, San Diego, CA.

9.     2018/5/6-11, Tailoring antibody glycosylation via integrating genome and protein engineering to generate preferred glycoforms on the Fc region, Cell Culture Engineering XVI, Tampa, FL. (oral)

10.  2017/08/20-24, Sialic acid modification: stabilization and determination of linkages of sialylated glycans, 254th American Chemical Society National Meeting & Exposition, Washington DC. (oral)

11.  2017/6/4-9, Identification of sialic acid linkages using esterification on solid-phase, 65th conference on Mass Spectrometry and Allied Topics, Indianapolis, IL.

12.  2017/5/5, Identification of sialic acid isomers from influenza virus replications in MDCK cells using solid-phase chemoenzymatic method, 2017 NIH&FDA Glycoscience Research Day, Bethesda, MD. (oral)

13.  2017/03/5-9, Career development often driven by the opportunity and persistence, CACA Pittcon Conference 2017, Chicago, IL (oral)

14.  2016/06/5-9, N-glycan analysis using GIG-automation on sialylglycotransferase knock-in CHO cell line, 64th Conference on Mass Spectrometry and Allied Topics, San Antonio, TX.

15.  2016/06/5-9, QUANTITY: An isobaric tag for quantitative glycomics, 64th Conference on Mass Spectrometry and Allied Topics, San Antonio, TX. (oral)

16.  2016/06/5-9, GPQuest: a free software for identification of glycans and intact glycopeptides, 64th Conference on Mass Spectrometry and Allied Topics, San Antonio, TX.

17.  2016/06/29, Glycan analysis using GIG-automation for biological applications, 2016 NIH&FDA Glycoscience Research Day, Bethesda, MD.

18.  2016/06/23, Quantitative analysis of N-glycans in glycoengineered EPO using QUANTITY, 2016 Biomanufacturing Technology Summit, Rockville, MD.

19.  2016/03/13-16, A novel tandem isobaric tag for quantitative analysis of N-glycans: QUANTITY, US HUPO, Boston, MA. (oral)

20.  2016/03/4-7, A role for glycans in Bla g 2 cockroach allergen-induced allergic responses, 2016 AAAAI Annual Meeting, Los Angeles, CA.

21.  2016/01/15, Signature proteins associated with lung carcinomas, PEG Meeting, Baltimore, MD. (oral)

22.  2015/07/30-31, Glycomic analysis using chemoenzymatic platform, Advanced Mammalian Biomanufacturing Innovation Center NSF I/UCRC Planning Grant Meeting, Mt. Washington, Baltimore, MD.

23.  2015/07/24, Mass spectrometry core: mass spectrometric analyses of glycans, PEG in Glycosciences, Annual Retreat, Mt. Washington, Baltimore, MD. (oral)

24.  2015/05/31-06/04, Glycoproteomic and proteomic analyses of hearts from hypertrophic cardiomyopathy, 63rd ASMS Conference on Mass Spectrometry and Allied Topics, St Louis, MO.

25.  2015/04/17, Regulation of proteins in left-ventricular dyssynchronous heart by cardiac resynchronization therapy, The NHLBI / JHU Proteomics Retreat, Baltimore, MD (oral).

26.  2014/10/10-14, The use of QABIT-ESI-HPLC-MS/MS for quantitation of tissue sialylation in a mouse model of GNE myopathy, 30th Asilomar Conference on Mass Spectrometry: Advanced in glycomics and glycoproteomics, methods and applications, Pacific Grove, CA. (oral)

27.  2014/9/8-10, Glycomic analysis of prostate cancer tissue and serum, 9th Early Detection Research Network (EDRN) Scientific Workshop, Bethesda, MD.

28.  2014/06/15-19, Identification and quantitation of N-glycans in dyssynchronous heart failure, 62nd ASMS Conference on Mass Spectrometry and Allied Topics, Baltimore, MD.

29.  2014/05/19, Glycan and protein analysis of mouse tissue using chemoenzymatic immobilization and mass spectrometry, NIH & FDA Glycosciences Research Day, Bethesda, MD. (oral)

30.  2014/03/26-28, Quantitative analysis of N-glycosylation in dyssynchronous heart failure, Inter PEG 2014, Bethesda, MD. (oral)

31.  2013/06/27, Glycomics analysis using solid-phase glycan extraction on chip and chipLC-MS, Glyco22, Dalian, China. (oral)

32.  2012/9/9, Glycomics analysis using chemical immobilization of glycans, solid-phase glycan modifications, and tandem mass spectrometry, Boston, MA.

33.  2012/5/1, Solid-phase glycan extraction for glycoprotein analysis, The NHLBI / JHU Proteomics Retreat, Baltimore, MD. (oral)

34.  2012/4/26, Glycopeptide, glycosite, and glycan analysis by solid-phase extraction, Baltimore, MD.

35.  2012/5/8, Glycan analysis by solid-phase capture and release using reversible reaction to hydrazide, 1st Annual Area-wide Glycobiology Poster Session, Baltimore, MD.

36.  2012/3/27, Novel fabrication process for transverse and longitudinal mode PZT actuators, Proceedings of Mid-Atlantic Micro/Nano Alliance Spring Symposium, Annapolis, MD.

37.  2012/2/25, Glycan analysis by reversible reaction to solid support and mass spectrometry, 7th Annual Johns Hopkins Prostate Research Day, Baltimore, MD.

38.  2012/3/27, Traveling wave annular ultrasonic micromotors using bulk PZT, Microsystems for Measurement and Instrumentation (MAMNA), Annapolis, MD.

39.  2012/3/27, Novel fabrication process for transverse and longitudinal mode PZT actuators, Microsystems for Measurement and Instrumentation (MAMNA), Annapolis, MD.

40.  2011/11/10, Glycan analysis by solid-phase capture and release using reversible reaction to hydrazide, Seattle, WA.

41.  2011/9/14, Hydrazide coated chip for capture and on target analysis of glycans, 7th EDRN Scientific Workshop, Bethesda (highlight poster).

42.  2011/6/9, Glycoproteomics identification and integration, presented to ADA Technologies, Inc., Denver, CO. (Oral)

43.  2011/6/5, Hydrazide coated chip for capture and on target analysis of glycans, ASMS 2011, Denver, CO. (Oral)

44.  2011/6/15, Hydrazide coated chip for glycan imaging,” NIH&FDA Glycoscience Research Day, Bethesda, MD.

45.  2011/3/14, Lab-on-a-chip for glycoprotein analysis, Johns Hopkins University National Heart Lung Brain Institute (NHLBI) Proteomic Innovation Retreat on Heart Failure, Baltimore

46.  2009/6/21, Microfluidic two-dimensional IEF-CZE for proteomics, Transducers 2009, Denver, CO. (Oral)

47.  2008/5/10, Protein two-dimensional separation on spatially-multiplexed polymer microchip platform, Proceedings of 32ndInternational Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC), Baltimore, MD

48.  2008/10/12, Spatially-multiplexed protein separation enabled by multifunctional photopolymerized gels, MicroTAS 2008, San Diego, CA.

49.  2006/11/5, Automated two-dimensional microfluidic protein profiling combining IEF and SDS gel electrophoresis, MicroTAS 2006, Tokyo, Japan.(Oral)

50.  2005/9/26, Electrochemical migration of land grid array sockets under highly accelerated stress conditions, IEEE 51st Holm Conference, Chicago, IL. (Oral)

 

Teaching

 

Fall/2007           Teaching assistant on course “Electronics and Instrumentation”

Summer/2013    Lab teaching on N-glycan analysis on course “Techniques in Glycobiology”

                        Lecture on N-glycan analysis using solid-phase

                        Hand-on experiments on N-glycan analysis using GIG

                        Mass spectrometry using MALDI-MS/MS

                        Data analysis on MS and report

Summer/2015    Lab teaching on N-glycan and O-glycan analysis on course “Techniques in Glycobiology”

Lecture on N-glycan analysis using Automation

                        Hand-on experiments on N-glycan analysis using GIG Automation

                        Mass spectrometry using MALDI and ESI MS/MS

2012-2015         Train post-docs, graduate students and summer interns for sample preparation (glycomics and glycoproteomics), mass spectrometry analysis, and data analysis     

 

Extramural Funding (current and previous)

CNSF 81871955: Mechanism of abnormal glycosylation modification on gastric cancer development caused by increased expression of β-1,4 galactosyltransferase 3.

Role: Glycosylation and proteomics (Principal Investigator: S. Yang)

 

P01HL107153 (NIH/NHLBI): Glycoconjugates and cardiovascular disease (2011-2018)

Role: Involve in glycoproteomic and glycomics (pI: Zhang H; co-Investigator: S. Yang)

Notes: This is a center of Programs of Excellence in Glycosciences (PEG). The central theme of this center is the roles of both extracellular and intracellular glycoconjugates in the mechanisms protecting the heart or leading to atherosclerosis and cardiomyopathies, culminating in myocardial infarction and heart failure.

 

HHSN268201000032C (N01-HV-00240) (NIH/NHLBI): Johns Hopkins proteomic innovation center in heart failure (2010-2015)

Role: Glycoproteomics and glycomics (Co-pI: Zhang H; co-Investigator: S. Yang)

Notes: This is a center of NHLBI Proteomics Centers. The goal of this project is to develop and apply innovative mass spectrometry based PTM capture methods in heart failure. In particular, this project will focus on glycoproteome tools in both tissue and blood samples.

 

U24CA115102 (NIH/NCI): Clinical and analytical validation of cancer biomarkers (2010-2015)

Role: Glycoprotein biomarker validation (pI: Chan DW; co-Investigator: H. Zhang)

Note: This is a biomarker reference laboratory (BRL) of the Early Detection Research Network (EDRN). The major goal of this project is to conduct analytical and clinical validation studies of biomarkers.

 

U01CA152813 (NIH/NCI): Glycoprotein biomarkers for the early detection of aggressive prostate cancer (2012-2015)

Role: Glycan analysis (pI: Zhang H; co-Investigator: S. Yang)

Notes: This is a biomarker developmental laboratory (BDL) of the Early Detection Research Network (EDRN). The goal of this project is to identify glycoprotein biomarkers for the early detection of aggressive prostate cancer in tissues and serum.

 

Maryland Innovation Initiative (MII): QUANTITY: A novel analysis kit for robust drug development, clinical diagnostics, and biomarker discovery (2015-2016)

Role: Kit development (pI: Zhang H, Li SW; co-pI: S. Yang)

Note: Developing a novel technology terms termed quaternary amine containing isobaric tag for glycan (abbreviated as QUANTITY) and its applications for drug development, clinical diagnostics, and biomarker discovery.

 

1R21CA191715-01 (NIH/NCI): AGX1/2 Inhibitors as Key Modulators of the Hexosamine Biosynthetic Pathway (2014-2017)

Role: Co-investigator (pI: Yarema KJ; co-pI: H. Zhang; co-Investigator: S. Yang)

Note: This project aims to study the roles of AGX1/2 Inhibitors in the Hexosamine Biosynthetic Pathway.

 

CBET 1512265 (NSF/ENG/CBET): Integrating novel nutrient feeding strategies with computational glycosylation models to improve production of complex biotherapeutics from mammalian factories (2015-2018)

Role: Co-investigator (pI: Betenbaugh M, Yarema KJ, Zhang H; co-Investigator: S. Yang)

Note: This project aims to use computational glycosylation models to improve production of complex biotherapeutics from mammalian cells.

 

ORGANIZATIONAL ACTIVITIES

2017/2018: NIH&FDA Glycoscience Research Day poster judge

2019: NIH&FDA Glycoscience Research Day session chair (cancer disease)

2018: ACS National Fall Meeting, Analytical Chemistry Session Chair (Glycobiology)

2016-2019: CASMS Connection Committee Member

2019: CASMS Executive Committee Member

 

Journal peer review activities

2007-present     IEEE Transactions on Device and Materials Research 

2007-present     IEEE Transactions on Advance Packaging

2010-present     IEEE Journal of MEMS   

2010-present    The Electrochemical Society Journal

2011-present     Journal of Proteome Research

2011-present    Electrophoresis

2001-present    Molecular BioSystems

2011-present    Journal of Separation Science

2011-present     Rapid Communication in Mass Spectrometry

2011-present    Journal of Chromatography A

2012-present    Analytical Chemistry

2012-present    Clinical Proteomics

2018-present      Journal of Hematology & Oncology

2018-present   The Journal of Pathology

2018-present   Chemical Science

2018-present   ChemComm

2019-present   Analytica Chimica Acta

2019-present   Genomics Proteomics & Bioinformatics

 

Professional Societies  

2003-2011      IEEE

2010-present  American Society for Mass Spectrometry (ASMS)

2010-present  American Chemical Society (ACS)

2011-present  Society for Glycobiology (SfG)

2011-present  World Human Proteomic Organization (HUPO) and USHUPO

2015-present  Chinese American Chromatography Association (CACA)