[12-14]Metabonomics delineates allergic reactions induced by Shuang--huang-lian injection in rats using ultra performance liquid chromatography-mass spectrometry

报告题目：Metabonomics delineates allergic reactions induced by Shuang-­huang-lian injection in rats using ultra performance liquid chromatography-mass spectrometry

报 告 人：顾媛媛（黑龙江中医药大学）

报告时间：2018年12月14日（星期五）上午9:00

报告地点：独墅湖校区二期云轩楼2301室

报告人简介：

Yuanyuan Gu held her PhD in Pharmacy from Heilongjiang University of Traditional Chinese Medicine in June 2013. During the doctoral period, 50 natural chemical components were extracted and isolated from the Impatiens balsamina L. Four compounds were screened by cell experiments and have certain application value for the treatment of cancer. During the post-doctoral period, through the metabonomics study of allergic reactions induced by Shuanghuanglian injection, 15 potential biomarkers related to allergic reactions were identified, and the metabolic pathways involved were used to explore the allergic reaction of Shuanghuanglian to rats. Currently, as an associate researcher, she is mainly engaged in the research of the traditional Chinese medicine for anti-cancer compound and the development of large health products.

报告摘要：

In this present study, metabonomics technology was used to explore the changes in endogenous metabolites in urine of the rat model of SHLI induced allergic reaction; we and analyzed the metabolites, metabolic pathway, and the mechanism which were closely related to the allergic reactions. The levels of serum histamine and tryptase were examined and changes in histomorphology were also observed. Based on the UPLC-Q-TOF/MS metabonomics, we carried out the pattern recognition analysis, selected potential biomarkers associated with allergic reactions, and explored the pathological mechanism for SHLI induced allergic reaction, which laid the foundation for the safety research of SHLI. Our results showed that SHLI increased the levels of serum histamine and tryptase in rats with allergic reaction; we determined 15 biomarkers in rat allergic reaction model induced by SHLI and found multiple metabolic pathways involved, such as metabolism of linolenic acid , phenylalanine , amino acid, 2-oxo acid, and purine and other metabolic pathways.