张真庆教授课题组

发布者:金雪明发布时间:2020-02-26浏览次数:4256



姓名:张真庆

职称:苏州大学特聘教授,江苏特聘教授

联系电话:0512-65882593

电子邮箱:z_zhang@suda.edu.cn

办公地址:苏大独墅湖校区2云轩楼1129

 

张真庆教授简介


一、学习工作经历

1、学历背景

1996.09 - 2000.06:中国海洋大学,药学

2000.09 - 2006.06:中国海洋大学,工学博士,研究方向糖科学

2、工作经历

2006.08 - 2009.09:美国Rensselaer Polytechnic Institute, 博士后

2009.10 –2012.08:美国Baxter Healthcare,研究员

2012.08 – 目前:  苏州大学药学院,教授

 

二、研究方向


1糖药物结构、制备工艺、质量控制

2、糖药物-免疫学构效关系研究

3、糖药物-神经退行性疾病构效关系研究

4、肝素类药物糖组学研究

5、抗体药物糖基化研究

 

三、主要研究成果

1、论文

(1) J. Hao, F. Wu, R. Tang, Y. Sun, D. Liu,*Z. Zhanga* Preparation of 1,4-linked α-D-glucuronans from starch with 4-acetamide-TEMPO/NaClO2/NaClO system,  International Journal of Biological Macromolecules,2020 in press. 2, IF4.8

(2)D. Bi, X. Li, T. Li, X. Li, Z. Lin, L. Yao, H. Li, H. Xu, Z. Hu, Z. Zhang*, Q. Liu* and X Xu* Characterization and Neuroprotection Potential of Seleno-Polymannuronate, Frontiers in Pharmacology, 2020,11: 21, 2区,IF3.8

(3)Y. Meng, L. Yi, L. Chen, J. Hao, D. Li, J. Xue*, N. Xu, Z.Zhang*. Purification, structure characterization and antioxidant activity of polysaccharides from Saposhnikovia divaricate, Chinese Journal of Natural Medicines.2019, 17(10): 0792-0800, 4IF:  1.991

(4)Y. Ouyang, M. Zhu, X.Wang, L. Yi, Y. Yao, J. Fareed, R. J Linhardt*, Z. Zhang,*Systematic analysis of enoxaparins from different sources with online one and two-dimensional chromatography, Analyst. 2019, 144, 3746-3755. 2区,IF 4.019

(5)Y. Ouyang, Y. Yu, F. Zhang, J. Chen, X. Han, K. Xia, Y.Yao, Z. Zhang,* R.J. Linhardt*, Non-Anticoagulant Low Molecular Weight Heparins for Pharmaceutical Applications, Journal Medicinal Chemistry, 2019, 62, 1067-1073. 2区,IF 6.054.

(6)Y. Ouyang, X. Han, Q. Xia, J. Chen, S. Velagapudi, K. Xia,Z. Zhang* and R.J Linhardt* Negative-ion Mode Capillary Isoelectric Focusing-Mass Spectrometry for Charge-Based Separation of Acidic Oligosaccharides, Analytical Chemistry, 2019, 91, 846-853. 1区,IF 6.35

(7)R. Cheng, X. Tang, A. Long, J. Mwangi, R. Lai, R. Sun, C. Long, Z. Zhang*. Purification and characterization of a novel anti-coagulant from the leech Hirudinaria manillensis. Zoological Research. 2019, 40(3): 205-210.

(8)F. WuK. Dong, M. Zhu, Q. Zhang, B. Xie, D. Li, H.Gan, Z. Zhang* Development of a method to analyze the complexes of enoxaparin and plateletfactor 4 with size-exclusion chromatography, Jounral of Pharmaceutical and Biomedical Analysis, 2019, 164, 668-671. 3区,IF 2.983

(9)Y. Ouyang, X. Han, Y. Yu, J. Chen, L. Fu, F. Zhang, R. Linhardt, J. Fareed, D. Hoppensteadt, W. Jeske, A. Kouta, Z. Zhang,* K. Xia*. Chemometric analysis of porcine, bovine and ovine heparins, Jounral of Pharmaceutical and Biomedical Analysis, 2019, 164, 345-352. 3区,IF 2.983

(10)Y. Lin, Q. Zhang, Y. Meng, J. Hao, B. Xie, H. Gan, D. Li, K. Dong, Z. Zhang*  Qualitative and quantitative analysis of 2, 5-anhydro-D-mannitol in low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry, Journal of Chromatography A. 2018, 1569:160-167. 2区,IF 3.858

(11)Z. Liu, Y. Xiong, L. Yi, R. Dai, Y. Wang, M. Sun, X. Xiao, Z. Zhang*, S. Yuan*, Endo-β-1,3-glucanase digestion combined with the HPAEC-PAD-MS/MS analysis reveals the structural differences between two laminarins with different bioactivities, Carbohydrate Polymers, 2018, 194, 339-349. 2区,IF: 6.044

(12)R. Tang1, J. Hao1, R.Zong, F. Wu, Y. Zeng, Z. Zhang*, Oxidation pattern of curdlan with TEMPO-mediated system, Carbohydrate Polymers, 2018, 186, 9-16. 2区,IF: 6.044

(13)Y. Ouyang, Y. Zeng, L. Yi, H. Tang, D. Li, R. Linhardt, Z. Zhang* Qualitative and quantitative analysis of heparin and low molecular weight heparins using size exclusion chromatography with multiple angle laser scattering/refractive index and inductively coupled plasma/mass spectrometry detectors, Journal of Chromatography A. 2017, 1522(3): 56-61, 2区,IF 3.858

(14)L. Xu, Z. Zhang*, X. Sun, J. Wang, W. Xu, L. Shi, J. Lu, J. Tang, J. Liu, X. Su.Glycosylation status of bone sialoprotein and its role in mineralization, Exp Cell Res. 2017 (17):30514-1. 2区,IF 3.329

(15)Y. Rong, N Xu, B Xie, J Hao, L Yi, R Cheng, D Li, R. J. Linhardt, Z. Zhang,* Sequencing analysis of β-glucan from highland barley with high performance anion exchange chromatography coupled to quadrupole time – of – flight mass spectrometry, Food Hydrocolliods,2017, 73, 235-242. 2区,IF: 5.839

(16)X. Sun, L. Tao, L. Yi, Y. Ouyang, N. Xu, D. Li, R. Linhardt, Z. Zhang* N-glycans released from glycoproteins using a commercial kit andcomprehensively analyzed with a hypothetical database, Journal of Pharmaceutical Analysis, 2017, 7(2): 87-94.  IF: 4.44

(17)L. Tao, F. Song, N. Xu, D. Li, R.J. Linhardt, Z. Zhang* New Insights into the Action of Bacterial Chondroitinase AC I and Hyaluronidase on Hyaluronic Acid, Carbohydrate Polymers, 2017, 158: 85-92.2区,IF: 6.044

(18)J. Hao, S. Xu, N. Xu, D. Li, R.J Linhardt, Z. Zhang*  Impact of Degree of Oxidation on the Physicochemical Properties of Microcrystalline Cellulose Carbohydrate Polymers, 2017, 155, 483-490.2区,IF: 6.044

(19)J. Hao, J. Lu, N. Xu, R. J. Linhardt, Z. Zhang*Specific oxidation pattern of soluble starch with TEMPO-NaBr-NaClO system, Carbohydrate Polymers, 2016, 146:238-244, 2区,IF: 6.044

(20)Y. Ouyang, C. Wu, X. Sun, J. Liu, R. J. Linhardt, Z. Zhang*. Development of Hydrophilic-Interaction-Chromatography with Quadruple-Time-of-Flight Mass Spectrometry for Heparin and Low-Molecular-Weight-Heparin Disaccharide Analysis, Rapid communications in mass spectrometry, 2016,30,277-284. 4区,IF: 2.045

(21)L. Yi, Y. Ouyang, X. Sun,N. Xu, R. J. Linhardt, Z. Zhang* Qualitative and quantitative analysis of branches in dextran using high-performance anion exchange chromatography coupled to quadrupole time-of-flight mass spectrometry, Journal of Chromatography A., 2015, 1423:79-85. 2区,IF 3.858

(22)Y. Ouyang, Y. Zeng, Y. Rong, Y. Song, L. Shi, B. Chen, X. Yang, N. Xu, R. J. Linhardt, Z. Zhang* Profiling Analysis of Low Molecular Weight Heparins by Multiple Heart-Cutting Two Dimensional Chromatography with Quadruple Time-of-Flight Mass Spectrometry, Analytical Chemistry, 2015, 87(17): 8957-8963. 1区,IF 6.35

(23)C. Wu, J. Liu, Z. Zhang* Hydrophilic interaction liquid chromatography- quadrupole-time-of-flight mass spectrometry analysis of glycosaminoglycan oligosaccharides, Chinese Journal of Analytical Chemistry, 2015, 43(8):1198-1202.4区,IF: 0.931

(24)L. Yi, X Sun, K. Du, Y. Ouyang, C. Wu, N. Xu, R. J. Linhardt, Z. Zhang*, UP-HILIC-MS/MS to Determine the Action Pattern of Penicillium sp. Dextranase, Journal of American Society for Mass Spectrometry, 2015, 26, 1174-1185. 3区,IF: 3.202

(25)J. Lu, H. Yang, J. Hao, C. Wu, L. Liu, N. Xu, R. J. Linhardt, Z. Zhang*, Impact of hydrolysis conditions on the detection of mannuronic to guluronic acid ratio in alginate and its derivatives,Carbohydrate Polymers, 2015, 122, 180–188, 2区,IF: 6.044

(26)Z. Zhang, S. Till,  S. Knappe, C. Quinn, J. Catarello, G. J. Ray, F. Scheiflinger, C. M. Szabo, M. Dockal*, Screening of Complex Fucoidans from Four Brown Algae Species as Procoagulant Agents Carbohydrate Polymers, Carbohydrate Polymers.2015,115, 677-685, IF: 2区,IF: 6.044


2、已授权专利

 

1. 不饱和透明质酸奇数寡糖及其制备方法,CN201611020047.X排名1

2. 一种氧化型β-1,4-寡聚葡萄糖醛酸及其制备方法和用途,CN201611020047.X排名1

3. 一种氧化型α-1,4-寡聚葡萄糖醛酸及其制备方法和用途,201510267211.6排名1

4. Factor VIII half-life extension by polysialic acid13/803,594排名2

5. Drug candidate screening and structure-activity relationship of a non-anticoagulant sulfated polysaccharide61/683,144排名3






李笃信 苏州大学药学院副教授(duxin.li@suda.edu.cn

李笃信副教授于2005年、2007年、2011年分别获得山东理工大学工学学士、南京理工大学 应用化学的工学硕士、华东理工大学分析化学的理学博士。20122015年赴德从事博士后研究,2015年受聘于苏州大学药学院副教授至今,发表SCI论文17篇,已授权专利8项,先后主持和参与国家博士后项目、国家自然科学基金面(面上)、省重点研发项目、企业课题等多个项目。


主要研究方向:


 1.二维液相色谱(LCxLC)新方法新技术开发;

 2.天然产物多糖的纯化、表征及其药效学评价;

 3.基于肠道菌群的中药代谢组学和药代动力学研究


近五年承担的科研项目:

1.61批国家博士后项目(7131701117),基于二维液相质谱的骨唾液酸蛋白质糖组学研究,2018/01-2019/12,主持。

2.国家自然科学基金面上项目(81673388),肝素类药物的糖组学研究,2017/01-2020/12,参与。

3.江苏省科学技术厅重点研发计划(社会发展),高值栀子黄及其副产物综合加工关键技术研究及产品开发,2019/01–2021/12, 参与。

4.苏州市重点产业技术创新,豆制品加工过程废弃物的资源化利用关键技术研发及产业化,2019/06-2021/06, 子课题负责人。

5.企业课题,siRNA序列确证方法学研究,2017.1 - 2019.6,主持。

6.企业课题,DNA序列确证方法学研究, 2018.8 - 2020.8,主持。

7.企业课题,制备型二维液相色谱系统开发,2019.4-2022.03,在研。

近五年发表论文:

1.F. Wu, K. Dong, M. Zhu, Q. Zhang, B. Xie, D. Li, H. Gan, R.J. Linhardt, Z. Zhang*, Development of a method to analyze the complexes of enoxaparin and platelet factor 4 with size-exclusion chromatography, Journal of pharmaceutical and biomedical analysis, 2019,164, 668-671.

2.L. Yi, Q. Zhang, Y. Meng, J. Hao, B. Xie, H. Gan, D. Li, K. Dong, Z. Zhang*, Qualitative and quantitative analysis of 2, 5-anhydro-D-mannitol in low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry, Journal of Chromatography A, 2018, 1569, 160-167.

3.X. Sun, L. Tao, L. Yi, Y. Ouyang, N. Xu, D. Li*, R.J. Linhardt, Z. Zhang*, N-glycans released from glycoproteins using a commercial kit and comprehensively analyzed with a hypothetical database, Journal of pharmaceutical analysis, 2017, 7, 87-94.

4.L. Tao, F. Song, N. Xu, D. Li, R.J. Linhardt, Z. Zhang*, New insights into the action of bacterial chondroitinase AC I and hyaluronidase on hyaluronic acid, Carbohydrate polymers, 2017, 158, 85-92.

5.Y. Rong, N. Xu, B. Xie, J. Hao, L. Yi, R. Cheng, D. Li, R.J. Linhardt, Z. Zhang*, Sequencing analysis of β-glucan from highland barley with high performance anion exchange chromatography coupled to quadrupole time–Of–Flight mass spectrometry, Food Hydrocolloids, 2017, 73, 235-242.

6.Y. Ouyang, Y. Zeng, L. Yi, H. Tang, D. Li, R.J. Linhardt, Z. Zhang*, Qualitative and quantitative analysis of heparin and low molecular weight heparins using size exclusion chromatography with multiple angle laser scattering/refractive index and inductively coupled plasma/mass spectrometry detectors, Journal of Chromatography A, 2017, 1522, 56-61.

7.J. Hao, S. Xu, N. Xu, D. Li, R.J. Linhardt, Z. Zhang*, Impact of degree of oxidation on the physicochemical properties of microcrystalline cellulose, Carbohydrate polymers, 2017, 155, 483-490.

8.D. Li, L. Gan, A. Bronja, O.J. Schmitz*, Gas chromatography coupled to atmospheric pressure ionization mass spectrometry (GC-API-MS), Analytica chimica acta, 2015, 891, 43-61.

9.D. Li, O.J. Schmitz*, Comprehensive two-dimensional liquid chromatography tandem diode array detector (DAD) and accurate mass QTOF-MS for the analysis of flavonoids and iridoid glycosides in Hedyotis diffusa, Analytical and bioanalytical chemistry, 2015, 407, 231-240.

10.D. Li, C. Jakob, O. Schmitz*, Practical considerations in comprehensive two-dimensional liquid chromatography systems (LCxLC) with reversed-phase in both dimensions, Analytical and bioanalytical chemistry, 2015, 407, 153-167.


已授权专利

1、一种无残留离心管,ZL201620807455.9,排名1

2、一种peek管割管器,ZL 201820074467.4,排名1

3、一种加压型快速透析装置,ZL 201821547791.X,排名1

4、一种蛋白糖基化分析装置,ZL 201820501388.7,排名1

5、一种高温全二维液相色谱装置及其使用方法,ZL 201710060507.X,排名1

6、不饱和透明质酸奇数寡糖及其制备方法,ZL 201611020047.X,排名3

7、以整体多孔材料为流道的液相色谱用的混合器及其方法,ZL 200920071840.1,排名2

8、一种在线全二维HILIC/RP液相色谱仪切换接口装置,ZL200810201040.7,排名2






徐乃玉 苏州大学药学院副教授(xunaiyu@suda.edu.cn

徐乃玉副教授于1997年获得沈阳药科大学药学学士学位,随后进入苏州大学任职,并于2003年、2008年取得苏州大学硕士、博士学位。主要研究方向是天然产物成分分析及其药效学评价。

近五年承担的科研项目

1.国家自然科学基金面上项目,81673388,肝素类药物的糖组学研究,2017/01-2020/12,参与。

2.国家自然科学基金面上项目,81473179,复杂蛋白聚糖的序列分析,2015/01-2018/12,参与。

3.江苏省自然基金面上项目,SBK201122569,野马追保护急性肺损伤的活性成分及作用机制研究2011/07-2014/07,参与。


近五年发表论文

1.N. Xu, C. Chu, L. Xia, J. Zhang, D. Chen*, Protective effects of Rabdosia japonica var. glaucocalyx extract on lipopolysaccharide-induced acute lung injury in mice, Chinese Journal of Natural Medicines, 2015, 10, 767-775.

2.Q. Sun, N. Xu, Q. Li, S. Yao, M. Li, H. Li, J. Zhang, D. Chen*, Antiglycemic and anticomplementary potential of an edible plant Gnaphalium hypoleucum DC. Journal of Functional Foods, 2017, 38, 321–328.

3.L. Tao, F. Song, N. Xu, D. Li, R.J. Linhardt, Z. Zhang*, New insights into the action of bacterial chondroitinase AC I and hyaluronidase on hyaluronic acid, Carbohydrate polymers, 2017, 158, 85-92.




欧阳艺兰 苏州大学药学院讲师(y_ouyang@suda.edu.cn), 分别于2013年、2016年、2019年获得黑龙江大学制药工程工学学士学位、苏州大学药物分析硕士和博士学位。2017-2018年赴美伦斯勒理工学院联合培养。主要研究方向是肝素类药物的结构分析与糖类药物的研究与开发。


近五年承担的科研项目

1.国家自然科学基金面上项目81673388),肝素类药物糖组学研究,2017.01-2020.12,参与。

2.国家自然科学基金面上项目(81473179,复杂蛋白聚糖的序列分析,2015.01-2018.12,参与。


近五年发表论文

1. Y. Ouyang, M. Zhu, X.Wang, L. Yi, Y. Yao, J. Fareed, R. J Linhardt*, Z. Zhang,* Systematic analysis of enoxaparins from different sources with online one and two-dimensional chromatography, Analyst. 2019, 144, 3746-3755.

2.Y. Ouyang, Y. Yu, F. Zhang, J. Chen, X. Han, K. Xia, Y.Yao, Z. Zhang,* R.J. Linhardt*, Non-Anticoagulant Low Molecular Weight Heparins for Pharmaceutical Applications, Journal Medicinal Chemistry, 2019, 62, 1067-1073.

3.Y. Ouyang, X. Han, Q. Xia, J. Chen, S. Velagapudi, K. Xia,Z. Zhang* and R.J Linhardt* Negative-ion Mode Capillary Isoelectric Focusing-Mass Spectrometry for Charge-Based Separation of Acidic Oligosaccharides, Analytical Chemistry, 2019, 91, 846-853.

4.Y. Ouyang, X. Han, Y. Yu, J. Chen, L. Fu, F. Zhang, R. Linhardt, J. Fareed, D. Hoppensteadt, W. Jeske, A. Kouta, Z. Zhang,* K. Xia*. Chemometric analysis of porcine, bovine and ovine heparins, Jounral of Pharmaceutical and Biomedical Analysis, 2019, 164, 345-352.

5.Y. Ouyang, Y. Zeng, L. Yi, H. Tang, D. Li, R. Linhardt, Z. Zhang* Qualitative and quantitative analysis of heparin and low molecular weight heparins using size exclusion chromatography with multiple angle laser scattering/refractive index and inductively coupled plasma/mass spectrometry detectors, Journal of Chromatography A. 2017, 1522(3): 56-61.

6.Y. Ouyang, C. Wu, X. Sun, J. Liu, R. J. Linhardt, Z. Zhang*. Development of Hydrophilic-Interaction-Chromatography with Quadruple-Time-of-Flight Mass Spectrometry for Heparin and Low-Molecular-Weight-Heparin Disaccharide Analysis, Rapid communications in mass spectrometry, 2016,30,277-284.

7.Y. Ouyang, Y. Zeng, Y. Rong, Y. Song, L. Shi, B. Chen, X. Yang, N. Xu, R. J. Linhardt, Z. Zhang* Profiling Analysis of Low Molecular Weight Heparins by Multiple Heart-Cutting Two Dimensional Chromatography with Quadruple Time-of-Flight Mass Spectrometry, Analytical Chemistry, 2015, 87(17): 8957-8963.