王剑文

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姓  名:王剑文

职称职务:教授

联系电话:69561430

电子邮箱:jwwang@suda.edu.cn

办公地址:云轩楼1425

 

 一、学习工作经历
1、学历背景:
198
3.91987.7 厦门大学生物系植物专业本科

1987.91990.7 厦门大学生物系植物生理学硕士

1998.92001.7 南京大学生命科学院植物化学(次生代谢调控方向)博士

2001.82003.7 南京大学环境科学院环境生物学博士后,副研究员

 

2、国外学习研究经历:
2003.8
2004.7 香港理工大学生物化学系研究助理(Research Associate

2008.102009.10 英国格拉斯哥大学生命科学院访问学者

3、工作经历:
1990.8
1998.9 中科院西双版纳热带植物园昆明分部(原中科院昆明生态研究所),助理研究员
2004.8
-苏州大学药学院 教授,博士生导师

二、研究方向
1)重要药用活性成分生物合成的调控研究

2)细胞防御反应中活性氧信号转导研究

3)药用植物、真菌的培养及活性成分生产

4)特境微生物的成分与功能研究

三、主要研究成果
主持承担了国家自然科学基金项目、江苏省自然科学基金等项目。在SCI刊物发表论文40余篇,作为副主编编写专著3部。获江苏省“333高层次人才培养工程”首批中青年科学技术带头人、江苏省“六大人才高峰”第五批高层次人才、苏州大学“东吴学者”称号。

1. 主要SCI(E)刊物论文 *为通讯作者)

1.          W. S. Pan, L. P. Zheng, H. Tian, W. Y. Li, J.W. Wang*. 2014. Transcriptome responses involved in artemisinin production in Artemisia annua L. under UV-B radiation. Journal of Photochemistry and Photobiology B: Biology 140: 292-300.

2.         Q. J. Wang, L. P. Zheng, P. F. Zhao, Y. L. Zhao, J.W. Wang*. 2014. Cloning and characterization of an elicitor-responsive gene encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase involved in 20-hydroxyecdysone production in cell cultures of Cyanotis arachnoidea. Plant Physiology and Biochemistry 84: 1-9.

3.          G. Z. Ao, X. J. Chu, Y.Y. Ji, J.W. Wang*. 2014. Antioxidant properties and PC12 cell protective effects of a novel curcumin analogue (2E,6E)-2,6-bis(3,5- dimethoxybenzylidene)cyclohexanone (MCH). International Journal of Molecular Sciences 15(3): 3970-3988.

4.          W.H.Yang, L.P. Zheng, H.Y.Yuan, J.W. Wang*. 2014. Glaucocalyxin A and B regulate growth and induce oxidative stress in lettuce (Lactuca sativa L.) roots. Journal of Plant Growth Regulation 33:384–396.

5.          Y.Y. Ji, Y.M. Zhu, J.W. Wang*.2013. GS-2, a pyrazolo[1,5-a]indole derivative with inhibitory activity of topoisomerases, exerts its potent cytotoxic activity by ROS generation. Environmental Toxicology and Pharmacology 36(3): 1186-1196.

6.          H.X. Li, Y. Xiao, L. L. Cao, X. Yan, C. Li, H.Y. Shi, J.W. Wang*, Y. H. Ye*. 2013. Cerebroside C increases tolerance to chilling injury and alters lipid composition in wheat roots. PLoS ONE 8(9): e73380.

7.          W.H.Yang, Z Zhang, Y.H. Sima, J. Zhang, J.W. Wang*. 2013. Glaucocalyxin A and B-induced cell death is related to GSH perturbation in huaman leukemia HL-60 cells. Anti-Cancer Agents in Medicinal Chemistry 13(8): 1280-1290.

8.          Q.J. Wang, L.P. Zheng, H.Y. Yuan, J.W. Wang*. 2013. Propagation of Salvia miltiorrhiza from hairy root explants via somatic embryogenesis and tanshinone content in obtained plants. Industrial Crops and Products 50: 648-653.

9.          J.W. Wang, J.Y. Wu. 2013. Effective elicitors and process strategies for enhancement of secondary metabolite production in hairy root cultures. Advances in Biochemcal Engineering/Biotechnology 134: 55-89.

10.       B. Zhang, L.P. Zheng, W.Y. Li, J.W. Wang*. 2013. Stimulation of artemisinin production in Artemisa annua hairy roots by Ag-SiO2 core-shell nanoparticles. Current Nanoscience 9(3): 363-370.

11.       Q.J.Wang, L.P. Zheng, Y.H. Sima, H.Y. Yuan, J. W. Wang*. 2013. Methyl jsamonate stimulates 20-hydroxyecdysone production in cell suspension cultures of Achyranthes bidentata. Plant Omics 6(2):116-120.

12.       W. Li, Y. Y. Ji, J. W. Wang*, Y. M. Zhu*. 2012. Cytotoxic activities and DNA binding properties of 1-methyl-7H-indeno[1,2-b]quinolinium-7-(4-dimethylamino) benzylidene triflate. DNA and Cell Biology 31(6): 1046-1053.

13.       L.W. Gao, J. W. Wang*. 2012. Antioxidant potential and DNA damage protecting activity of aqueous extract from Armillaria mellea. Journal of Food Biochemistry 36: 139-148.

14.       T. Zou, L. P. Zheng, H. Y. Yuan, Y. F. Yuan, J. W. Wang*. 2012. The nitric oxide production and NADPH-diaphorase activity in root tips of Vicia faba L. under copper toxicity Plant Omics 5:115-121.

15.       B. Zhang, L.P. Zheng, J. W. Wang*. 2012. Nitric oxide elicitation for secondary metabolite production in cultured plant cells. Applied Microbiology & Biotechnology 93: 455–466.

16.       L.W. Gao, J Zhang, W. H. Yang, B. Wang, J. W. Wang*. 2011. Glaucocalyxin A induces apoptosis in human leukemia HL-60 cells through mitochondria-mediated death pathway. Toxicology in Vitro 25: 51–63.

17.       C. Garcia-Mata, J.W. Wang, P Gajdanowicz, W. Gonzalez, W. Gonzalez, A. Hills, A. N. Donald, J. Riedelsberger, Amtmann, I. Dreyer, M. R. Blatt. 2010. A minimal cysteine motif required to activate the SKOR K+ channel of Arabidopsis by the reactive oxygen species H2O2. Journal of Biological Chemistry 285: 29286-29294.

18.       J. W. Wang, J. Y. Wu. 2010. Tanshinone biosynthesis in Salvia miltiorrhiza and production in plant tissue cultures. Applied Microbiology & Biotechnology 88:437–449.

19.       L. P. Zheng, B. Zhang, T. Zou, Z. H. Chen, J. W. Wang*. 2010. Nitric oxide interacts with reactive oxygen species to regulate oligosaccharide-induced artemisinin biosynthesis in Artemisia annua hairy roots. Journal of Medicinal Plants Research 4: 758-765.

20.       J. W. Wang*, L. P. Zheng, Zhang B, Zou T. 2009. Stimulation of artemisinin synthesis by combined cerebroside and nitric oxide elicitation in Artemisia annua hairy roots. Applied Microbiology and Biotechnology 85:285–292.

21.       J.Q. Zhou, J. W. Wang*. 2009. Immobilization of alliinase with a water soluble-insoluble reversible N-succinyl-chitosan for allicin production. Enzyme and Microbial Technology. 45: 299–304.

22.       L. P. Zheng, Y. T. Guo, J. W. Wang*, R. X. Tan. 2008. Nitric oxide potentiates oligosaccharide-induced artemisinin production. Journal of Integrative Plant Biology 50: 49–55.

23.       J. W. Wang, L. P. Zheng, R. X. Tan. 2007. Involvement of nitric oxide in cerebroside-induced defense responses and taxol production in Taxus yunnanensis suspension cells. Applied Microbiology and Biotechnology 75: 1183-1190.

24.       J. W. Wang, L. P. Zheng, J. Y..Wu, R. X. Tan. 2006. Involvement of nitric oxide in oxidative burst, phenylalanine ammonia-lyase activation and Taxol production induced by low-energy ultrasound in Taxus yunnanensis cell suspension cultures. Nitric Oxide-Biology and Chemistry 15: 351-358.

25.       J. W. Wang, J. H. Wu, W. Y. Huang, R. X. Tan. 2006. Laccase production by Monotospora sp., an endophytic fungus in Cynodon dactylon. Bioresource Technology 97: 786–789.

26.       J. W. Wang, J. Y. Wu. 2005. Nitric oxide is involved in methyl jasmonate-induced defense responses and secondary metabolism activities of Taxus cells. Plant and Cell Physiology 46: 923–930. 

27.       J. W. Wang, J. Y. Wu. 2004. Involvement of nitric oxide in elicitor-induced defense responses and secondary metabolism of Taxus chinensis cells. Nitric Oxide-Biology and Chemistry 11: 298-306.            

28.       Y. M. Yang, J. W. Wang, R.X. Tan. 2004. Immobilization of glucose oxidase on chitosan–SiO2 gel. Enzyme and Microbial Technology 34:126–131.

29.       J. W. Wang, Z. H. Xia, J. H. Chu, R. X. Tan. 2004. Simultaneous production of anthocyanin and triterpenoids in suspension cultures of Perilla frutescens. Enzyme and Microbial Technology 34: 651-656.     

30.       J. W. Wang, F. X. Kong, R. X. Tan. 2002. Improved artemisinin accumulation in hairy root cultures of Artemisia annua by (22S, 23S)-homobrassinolide. Biotechnology Letters, 24: 1573-1577.

31.       J. W. Wang, Z. H. Xia, R. X. Tan. 2002. Elicitation on artemisinin biosynthesis in Artemisia annua hairy roots by the oligosaccharide extract from the endophytic Colletotrichum sp. B501. Acta Botanica Sinica, 44(10): 1233-1238.

32.       J. W. Wang, R. X. Tan. 2002. Artemisinin production in Artemisia annua hairy root cultures with improved growth by altering the nitrogen source in the medium. Biotechnology Letters, 24: 1153-1156.     

33.       J. W. Wang, Z. Zhang, R. X. Tan. 2001. Stimulation of artemisinin production in Artemisia annua hairy roots by the elicitor from the endophytic Colletotrichum sp. Biotechnology Letters, 23(11), 857-860.

 

2. 课题                  

1.   国家自然科学基金项目(81473183)。活性氧在竹黄菌发育分化及竹红菌素合成中的调控作用研究。2015-2018.主持。

2.   国家自然科学基金项目(81273487)。内生菌对黄花蒿腺毛发育及其青蒿素合成的诱导调控研究。2013-2016.主持。

3.   国家教育部留学基金项目K513201011。一氧化氮对黄花蒿发根青蒿素生产的调控研究。2011-2013.主持。

4.   苏州大学“东吴学者计划”第二批高层次人才项目(14317363)。新型内生菌脑苷脂类信号对青蒿素生物合成的诱导构效和调控研究。2010-2015。主持。

5.   江苏省“六大人才高峰”第五批高层次人才项目(B类)。内生真菌脑苷脂类诱导物对青蒿素生产的调控研究。2009-2011。主持。

6.   国家自然科学基金项目(30772731)。一氧化氮介导黄花蒿内生真菌对宿主细胞青蒿素合成调控的研究。2008-2010.主持。

7.   江苏省自然科学基金面上项目(BK2007051)。黄花蒿内生真菌对宿主细胞一氧化氮的诱导及其与青蒿素合成的关系研究。2007-2009.主持。

8.   江苏省高校自然科学基金项目(05KJB360120)。一氧化氮在三种植物萜类药物生物合成诱导中的调控作用。2005-2007.主持。

9.   博士后科学基金项目。促进青蒿素合成的黄花蒿内生真菌诱导子活性成分研究. 2001-2003.主持.

 

3. 著作
1.谭仁祥主编、王剑文等副主编2003。《植物成分功能》,北京:科学出版社。已被国家教育部遴选为研究生教学用书。ISBN 7-03-011473-6

2.卢艳花主编、王剑文、魏东芝(副主编)2006。《天然药物的生物转化》,北京:化学工业出版社。ISBN: 7-50-258285-1

3.谭仁祥主编、王剑文副主编2009。《甾体化学》,北京:化学工业出版社。ISBN: 9-78-712203-624-7

(4). 专利

1. 王剑文、赵一璐、潘魏松、雷晓珩、陈可嘉。一种提高竹黄菌中竹红菌素产量的方法。(国家发明专利申请号201010530828.X),20102013124授权,专利号ZL 201010530828.X

2.谭仁祥、杨宇民、吴俊华、王剑文。一种病原微生物滤杀材料及其制法(国家发明专利申请号03131867.3),200320051026授权,专利号ZL 03131867.3

3.谭仁祥、纪莉莲、王蒙、王剑文。果蔬保鲜剂及其制法(国家发明专利申请号03132045.7),2003200614授权,专利号ZL 03132045.7

、主要荣誉

1.   谭仁祥、孔令东、罗兰、徐琛、王剑文、胡一桥、刘利根、唐海秋、郑维发、刘常宏、周长新. 民间常用草药成分的分析与功能研究. 教育部提名国家科学技术奖一等奖. 2005. 证书号2005-017

2. 王剑文. 江苏省“333高层次人才培养工程”首批中青年科学技术带头人。2007

3. 王剑文. 江苏省“六大人才高峰”第五批高层次人才。2009

4. 王剑文. 苏州大学“东吴学者计划”第二批高层次人才。2010

5. 王剑文. Stimulation of artemisinin synthesis by combined cerebroside and nitric oxide elicitation in Artemisia annua hairy roots。苏州市自然科学优秀学术论文。二等奖. 2010. 证书号SZNSP10152

6. 王剑文. 陈金荣生命科学英才奖(科研成果奖)二等奖。2009

7. 王剑文. 周氏教育科研奖(教学类)优秀奖2008

8. 王剑文. 陈金荣生命科学英才奖(科研成果奖)三等奖。2007

9. 王剑文、杨吉成、周建芹、章良、孙雄华。生物制药方向生物类课程教学改革与实际成果。苏州大学教学成果奖。二等奖.2007.

10. 王剑文. 红豆杉细胞中茉莉酸甲酯诱导的防卫反应、次生代谢活性与一氧化氮的关系研究。苏州市自然科学优秀学术论文。一等奖. 2006

11. 王剑文. 苏州大学陈金荣生命科学英才奖二等奖.2005.

12. 王剑文. 南京大学优秀博士后一等奖2003.

五、社会兼职
1. 中国植物学会植物化学与资源学专业委员会委员(2008-2018

2.〖抗感染药学〗编委(2005-

3. Research of Pharmaceutical Biotechnology 〗编委(2010-

4.International Journal of Medicinal and Aromatic Plants 〗编委 2011-

5. 〖生物过程〗编委(2011-