张真庆

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姓名:张真庆

职称:苏州大学特聘教授,江苏特聘教授

联系电话:0512-65882593

电子邮箱:z_zhang@suda.edu.cn

办公地址:苏大独墅湖校区2 云轩楼1129

 

张真庆教授简介

一、学习工作经历

1、学历背景

1996.09 - 2000.06:中国海洋大学,药学

2000.09 - 2006.06:中国海洋大学,工学博士,研究方向糖科学

2、工作经历

2006.08 - 2009.09:美国Rensselaer Polytechnic Institute, 博士后

2009.10 –2012.08:美国Baxter Healthcare,研究员

2012.08 – 目前:  苏州大学药学院,教授

 

二、研究方向

1、肝素及肝素类药物的分析,鉴定

2、糖蛋白和蛋白聚糖的糖基化分析

3、活性糖链的生物合成、衍生以及蛋白的多糖修饰研究

4、活性糖链与抗肿瘤和抗神经退行性疾病的构效关系研究

5、糖类药物的研究与开发

 

三、主要研究成果

张真庆教授以糖类药物为研究对象先后在Nat.Biotech, PNASJACSNPRJBC, Anal. Chem.JMC等杂志发表论文50余篇。总影响因子超过了190,截止到2012年总引次数超过了700。申请美国专利两项。 张真庆博士在糖化学糖生物学以及糖类药物的分析与开发领域获得了广泛的认可,有较高的知名度。

代表性论文(1 共同作者;* 通信作者)

1.   M. Guerrini, D. Beccati, Z. Shriver, A. Naggi, K. Viswanathan, A. Bisio, I. Capila, J.C. Lansing, S. Guglieri, B. Fraser, A. Al-Hakim, N.S. Gunay, Z. Zhang, L. Robinson, L. Buhse, M. Nasr, J. Woodcock, R. Langer, G. Venkataraman, R.J. Linhardt, B. Casu, G. Torri, R. Sasisekharan*. Oversulfated chondroitin sulfate is a contaminant in heparin associated with adverse clinical events, Nature Biotechnolology, 2008, 26(6): 669-675. 影响因子31

2.   M. Guerrini,1 Z. Zhang,1 Z. Shriver,1 A. Naggi , S. Masuko , R. Langer, B. Casu , R. J. Linhardt , G. Torri, R. Sasisekharan*, Orthogonal analytical approaches to detect potential contaminants in heparin, PNAS, 2009, 106, 16956-61. 1Co- first authors. 影响因子9.8

3.   Z. Zhang, S.A. McCallum, J. Xie, L. Nieto, F. Corzana, J. Jiménez-Barbero, M. Chen, J. Liu, R.J. Linhardt*. Solution Structures of Chemoenzymatically Synthesized Heparin and Its Precursors, Journal of the American Chemical Society, 2008, 130(39): 12998-3007.影响因子9.0

4.   H. Liu, Z. Zhang, R.J. Linhardt*. Lessons learned from the contamination of heparin, Natural Product Reports (NPR), highlight paper, 2009, 26(3):313-321.影响因子7.7

5.   Z. Zhang*, N. Khan, C.M. Szabo, Complete Monosaccharide Analysis by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection. Anal. Chem. 2012, 84, 4104-4110. 影响因子5.8

6.   Z. Zhang, J. Xie, H. Liu, J. Liu, R.J. Linhardt*. Quantitification of Heparan Sulfate Disaccharides Using Ion-pairing, Reverse-Phase, Micro-flow, High Performance Liquid Chromatography with Electrospray Ionization Trap Mass Spectrometry. Analytical Chemistry, 2009, 81, 4349-4355. 影响因子5.8

7.   Z. Zhang, M. Weïwer, B. Li, M.M. Kemp, T.H. Daman, R.J. Linhardt*. Oversulfated Chondroitin Sulfate: Impact of a Heparin Impurity, Associated with Adverse Clinical Events, on Low-Molecular-Weight Heparin Preparation. Journal of Medicinal Chemistry, 2008, 51(18): 5498-501. 影响因子5.2

8.   Z. Zhang, J. Xie, J. Liu, R.J. Linhardt*. Tandem MS can distinguish hyaluronic acid from N-acetylheparosan, Journal of the American Society of Mass Spectrometry, 2008, 19(1): 82-90. 影响因子3.8

9.   Z. Zhang, G. Yu, X Zhao, H. Liu, H Guan, A.M. Lawson W. Chai*. Sequence analysis of alginate-derived oligosaccharide by ESI/CID-MS/MS. Journal of theAmerican Society of Mass Spectrometry, 2006, 17, 621–630. “ SCI ” 影响因子3.8

10. Z. Zhang, J. Xie, F. Zhang, R.J. Linhardt*. Thin-layer chromatography for the analysis of glycosaminoglycan oligosaccharides. Analytical Biochemistry, 2007, 371(1): 118-120. “ SCI ” 影响因子3.3

11. Z. Zhang, Y. Park, M.M. Kemp, W. Zhao, D. Shaya, M. Cygler, Y.S. Kim, R.J. Linhardt*. Liquid chromatography–mass spectrometry to study chondroitin lyase action pattern, Analytical Biochemistry, 2009, 385(1): 57-64. 影响因子3.3

12. Z. Zhang, B. Li, J. Suwan, F. Zhang, R.J. Linhardt*. Analysis of pharmaceutical heparins and potential contaminants using 1H-NMR and PAGE, Journal of Pharmaceutical Science, 2009, 98(11):4017-4026. 影响因子3.0

13. Z. Zhang, R.J. Linhardt*. Sequence analysis of native oligosaccharides using negative ESI tandem MS, Current Anal. Chemistry, 2009, 5, 225-237. 影响因子2.1

14. Z. Zhang, G. Yu*, H. Guan, X. Zhao, Y. Du, X. Jiang, Preparation and structure elucidation of alginate oligosaccharides degraded by alginate lyase from Vibrio Sp510. Carbohydrate Research, 2004, 399,1475-1481. 影响因子2.0

15. Z. Zhang, Z. Xiao, R.J. Linhardt*. Thin Layer Chromatography for the Separation and Analysis of Acidic Carbohydrates, Journal of Liquid Chromatography & Related Technologies, 2009, 1711-1732. 影响因子1.0

16. Z. Wang, B. Yang, Z. Zhang, M. Ly, M. Takieddin, S. Mousa, J. Liu, J. S. Dordick, R.J. Linhardt* Control of the heparosan N-1 deacetylation leads to animproved bioengineered heparin. Applied Microbiology and Biotechnology, 2011, 91-99. 影响因子3.3

17. G. Yu, Y. Zhang, Z. Zhang, L. Song, W. Chai*, The Effect and Limitation of Excess Ammonium on the Release of O-Glycansin Reducing Forms from Glycoproteins under Mild Alkaline Conditions for Glycomic and Functional Analysis. Analytical Chemistry, 2010, 82, 9534-9542. 影响因子5.8

18. T. N. Laremore, M. Ly, Z. Zhang, K. Solakyildirim, S. McCallum, R. T. Owens, R. J. Linhardt*, LC-MS disaccharide composition analysis for the domain structure elucidation of decorin glycosaminoglycan. Biochemical Journal, 2010, 431:199-205. 影响因子5.2

19. Z. Xiao, W. Zhao, B. Yang, Z. Zhang, H. Guan, R. J. Linhardt*,  Heparinase susceptible 3,6-di-O-sulfo-2-deoxy-2- sulfamido- a-D-glucopyranose (1,4) 2-O-sulfo- a-L-idopyranosyluronic acid linkage. Glycobiology, 2011, 21: 13-22. “ SCI ” 影响因子3.9

20. D. Shaya, W. Zhao, M.L. Garron, Z. Xiao, Q. Cui, Z. Zhang, T. Sulea, R.J. Linhardt, M. Cygler, Catalytic mechanism of heparinase II investigated by site-directed mutagenesis and the crystal structure with its substrate. J. Biol. Chem, 2010, 25; 285(26):20051-61. 影响因子6.9

21. Z. Wang, Z. Zhang, SA McCallum, RJ Linhardt*, Nuclear magnetic resonance quantification for monitoring heparosan K5 capsular polysaccharide production. Analytical Biochemistry, 2010, 398, 275-277. 影响因子3.3

22. AR Im, Y Park, JS Sim, Z. Zhang, Z. Liu, RJ Linhardt, YS Kim*, Glycosaminoglycans from earthworms (Eiseniaandrei). Glycoconjugate Journal,  “ SCI ” 影响因子3.32010, 27(2):249-257. IF: 2.5

23. K. Solakyildirim, Z. Zhang, R. Linhardt*, Ultraperformance Liquid Chromatography with Electrospray Ion Trap Mass Spectrometry for Chondroitin Disaccharide Analysis. Analytical Biochemistry, 2010, 397: 24-28.影响因子3.3

24. S. Bhattacharyya, K. Solakyildirim, Z. Zhang, R. J. Linhardt, J. K. Tobacman*. Increase in Cell-Bound Interleukin-8 follows Silencing of N-Acetylgalactosamine-4-Sulfatase (Arylsulfatase B) and is Attributable to Increased Chondroitin 4-Sulfate. American Journal Respiratory Cell and Molelulor Biology, 2010, 42, 51-61. SCI ” 影响因子4.3

25. S. Bhattacharyya, H. Liu, Z. Zhang, G. Michel, R. J. Linhardt, J. Tobacman*. Carrageenan-Induced Innate Immune Response is Modified by Enzymes that Hydrolyze Distinct Galactosidic Bonds. Journal Nutritional Biochemistry, 2010, 21, 906–913. 影响因子4.3

26. S. Bhattacharyya, K. Solakyildirim, Z. Zhang, R.J. Linhardt, J.K. Tobacman*. Chloroquine reduces arylsulphatase B activity and increases chondroitin-4-sulphate: implications for mechanisms of action and resistance. Malaria Journal, 20098303   影响因子3.22009, 8(1):303. IF: 3.0 

27. Y. Han, M. Garron, H. Kim, W. Kim, Z. Zhang, K. Ryu, D. Shaya, Z. Xiao, C. Cheong, Y. Kim, R. Linhardt, M. Cygler*, Structural snapshots of heparin depolymerization by heparin lyase I. J. Biol. Chem. 2009, 284: 34019-34027.影响因子6.9

28. F. Zhang, Z. Zhang, X. Lin, A. Beenken, M. Mohammadi, R. J. Linhardt*, Compositional analysis on heparin/heparan sulfate interacting with FGF•FGFR complexes. Biochemistry, 2009, 48, 8379–8386. 影响因子3.2

29. B. Li, J. Suwan, J. G. Martin, F.Zhang, Z. Zhang, D. Hoppensteadt, M. Clark, J. Fareed, R. J. Linhardt* Oversulfated chondroitin sulfate interaction with heparin-binding proteins: New insights into adverse reactions from contaminated heparins. Biochemical Pharmacology, 2009, 72, 292-300. 影响因子4.3

30. J. Suwan, Z. Zhang, B. Li, P. Vongchan, P. Meepowpan, F.Zhang, S. A. Mousa, S. Mousa, B. Premanode, P. Kongtawelert, R. J. Linhardt*. Sulfonation of papain treated chitosan and its mechanism for anticoagulant activity. Carbohydrate Research, 2009, 344, 1190-1196.影响因子2.0

31. N. Volpi*, Z. Zhang, R.J. Linhardt. Mass spectrometry for the characterization of unsulfated chondroitin oligosaccharides from 2-mers to 16-mers. Comparison with hyaluronic acid oligomers. Rapid Communications in Mass Spectrometry, 2008, 22(22): 3526-30. 影响因子2.7

32. F. Zhang, Z. Zhang, R. Thistle, L. McKeen, S. Hosoyama, T. Toida, R.J. Linhardt*, P. Page-McCaw. Structural characterization of glycosaminoglycans from zebrafish in different ages. Glycoconjugate Journal, 2009, 26(2):211-218. 影响因子2.5

33. Y. Park, Z. Zhang, R.J. Linhardt, E.K. Lemosy*. Distinct heparan sulfate compositions in wild-type and pipe-mutant eggshell matrix. Fly, 2008, 2, 175 – 179. 影响因子1.2

34. Y. Park, Z. Zhang, T.N. Laremore, B. Li, J.S. Sim, A.R. Im, M.Y. Ahn, Y.S. Kim, R.J. Linhardt*. Variation of acharan sulfate and monosaccharide composition and analysis of neutral N-glycans in African giant snail (Achatinafulica). Glycoconjugate Journal, 2008, 25(9): 863-877. 影响因子2.5

35. A.I. de Agostini*, J.C. Dong, C. de VantéryArrighi, M.A. Ramus, I. Dentand-Quadri, S. Thalmann, P. Ventura, V. Ibecheole, F. Monge, A.M. Fischer, S. Hajmohammadi, N.W. Shworak, L. Zhang, Z. Zhang, R.J. Linhardt. Human follicular fluid heparan sulfate contains abundant 3-O-sufated chains with anticoagulant activity. J. Biol. Chem. 2008, 283(42): 28115-24. 影响因子6.9

36. M. Warda, F. Zhang, M. Radwan, Z. Zhang, N. Kim, Y.N. Kim, R.J. Linhardt, J. Han*. Is human placenta proteoglycan remodeling involved in pre-eclampsia? Glycoconjugate Journal, 2008, 25(5): 441-450. 影响因子2.5

37. A. Silipo, Z. Zhang, F.J. Cañada, A. Molinaro, R.J. Linhardt, J. Jiménez-Barbero*. Conformational analysis of a dermatan sulfate-derived tetrasaccharide by NMR, molecular modeling, and residual dipolar couplings. Chembiochem, 2008, 9(2):240-252. 影响因子3.8

38. X. Zhao*, G. Yu, H. Guan, N. Yue, Z. Zhang, H. Li. Preparation of low-molecular-weight polyguluronate sulfate and its anticoagulant and anti-inflammatory activities. Carbohydrate Polymers2007, 69(2): 272-279.影响因子3.2

39. L. Gan, X. Xin, J. Li, H. Liu, Z. Zhang, J. Qian, J. Ding, M. Geng*. Characterization of Conformational Epitope of Alginate-derived Poly- mannuronates by Surface Plasmon Resonance. Biochimie, 2005, 87, 959-66. 影响因子3.5

40. H. Liu, M. Geng, X. Xin, F. Li, Z. Zhang, J. Li, J. Ding*. Multiple and Multivalent Interactions of Novel Anti-AIDS Drug Candidates, Sulfated Polymannuronate (SPMG)-derived Oligosaccharides, with gp120, and their Anti-HIV Activities.Glycobiology, 2005, 15, 501-510. 影响因子3.9

41. F. Zhang, Z. Zhang, R. Linhardt*,Glycosaminoglycans, in Handbook of Glycomics: Glycoconjugate Structural Analysis, R.D. Cummings, M. Pierce (eds.) Academic Press/Elsevier, 2009, Chapter 3, 59-80.

42. B. Li, H. Liu, Z. Zhang, H. Stansfield, J. Dordick, R. Linhardt*. Analysis of glycosaminoglycans in stem cell glycomics. in Embryonic Stem Cell Therapy for Osteodegenerative Diseases. Nicole zur Nieden, Ed., Humana Press, Methods in Molecular Biology 2011, 690, 285-300.