王剑文课题组在Int. J. Mol. Sci.发表科研论文

Antioxidant Properties and PC12 Cell Protective Effects of a Novel Curcumin Analogue (2E,6E)-2,6-Bis(3,5-dimethoxybenzylidene)cyclohexanone (MCH)
GZ Ao, XJ Chu, YY Ji, JW Wang*
Int. J. Mol. Sci. 2014, 15, 3970-3988; (IF2013=2.339)

Abstract: The antioxidative properties of a novel curcumin analogue (2E, 6E)-2, 6-bis(3, 5-dimethoxybenzylidene)cyclohexanone (MCH) were assessed by several in vitro models, including superoxide anion, hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and PC12 cells protection from H2O2 damage. MCH displayed superior O2•- quenching abilities to curcumin and vitamin C. In vitro stability of MCH was also improved compared with curcumin. Exposure of PC12 cells to 150 M H2O2 caused a decrease of antioxidant enzyme activities, glutathione (GSH) loss, an increment in malondialdehyde (MDA) level, and leakage of lactate dehydrogenase (LDH), cell apoptosis, reduction in cell viability. Pretreatment of the cells with MCH at 0.63-5.00 M before H2O2 exposure significantly attenuated those changes in a dose-dependent manner. MCH enhanced cellular expression of transcription factor NF-E2-related factor 2 (Nrf2) at the transcriptional level. Moreover, MCH could mitigate intracellular accumulation of reactive oxygen species (ROS), the loss of mitochondrial membrane potential (MMP), and the increase of caspase 3 activity induced by H2O2. These results show that MCH protects PC12 cells from H2O2 injury by modulating endogenous antioxidant enzymes, scavenging ROS, activating the Nrf2 cytoprotective pathway and prevention of apoptosis.