化学生物学实验室(许国强 教授)

主要研究方向:

Ÿ  定量蛋白质组学

Ÿ  药物蛋白质组学

Ÿ  蛋白质泛素化和类泛素化修饰

Ÿ  蛋白质相互作用和功能

 

主要成员:

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许国强 苏州大学特聘教授,医学部药学院教授、博士生导师

邮寄地址:江苏省苏州市工业园区仁爱路199号苏大二期云轩楼1328室(邮编:215123

联系电话:0512-65882723

手机:18352447040

电子邮箱:gux2002@suda.edu.cn

 

学习经历:

1991.8-1996.7            复旦大学,本科(包括一年军训)

1996.8-1999.7            复旦大学,理学硕士

1999.8-2002.12          美国OhioAkron大学理学博士

 

研究经历:

2003.1-2005.12   美国纽约州康奈尔大学化学和化学生物系,博士后

2006.1-2011.7    美国纽约市康奈尔大学Weill医学院药理学系,博士后

2011.7-2013.2      美国纽约市康奈尔大学Weill医学院药理学系,助理研究教授

2013.2-至今     苏州大学特聘教授、医学部药学院博士生导师

 

主要研究成果:

主要从事生物大分子的翻译后修饰及功能的研究,建立了多种新颖独特的高通量方法研究蛋白质在信号传导通路中的翻译后修饰,并探索其生理功能。解决了长期以来蛋白质泛素化研究中缺乏高通量鉴定关键技术的难题。在Nat. Biotechnol.Nat. Commun.FASEB J.PNASJBCProteomics等杂志上发表论文近60篇。

 

代表性论文:

1.Duan, W.; Chen, S.; Zhang, Y.; Li, D.; Wang, R.; Chen, S.; Li, J.; Qiu, X.; Xu, G.* Protein C-terminal enzymatic labeling identifies novel caspase cleavages during the apoptosis of multiple myeloma cells induced by kinase inhibition. Proteomics 2016, 16, 60-69.

2.Liu, Y.; Huang, X.; He, X.; Zhou, Y.; Jiang, X.; Chen-Kiang, S.; Jaffrey, S.R.,* Xu, G.* A novel effect of thalidomide and its analogs: suppression of cereblon ubiquitination enhances ubiquitin ligase function. FASEB J. 2015, 29, 4829-4839.

3.Liu, Y.; He, X.; Sui, Y.; Yu, R.; Xu, G.* Transcription factor IKZF1 is degraded during the apoptosis of multiple myeloma cells induced by kinase inhibition. FEBS Lett. 2015, 589, 2233-2240.

4.Zhang, Z.; Zhou, L.; Zhou, Y.; Liu, J.; Xing, X.; Zhong, J.; Xu, G.; Kang, Z.; Liu, J.* Mitophagy induced by nanoparticle-peptide conjugates enabling an alternative intracellular trafficking route. Biomaterials 2015, 65, 56-65.

5.Sui, Y. #; Liu, Y.#; Xu, G.* A lysine-to-arginine mutation on NEDD8 markedly reduces the activity of cullin RING E3 ligase through the impairment of neddylation cascades. Biochem. Biophys. Res. Commun. 2015, 461, 653-658 (#: co-first authors).

6.Deglincerti, A.; Liu, Y.; Colak, D.; Hengst, U.; Xu, G.*; Jaffrey, S. R.* Coupled local translation and degradation regulate growth cone collapse. Nat. Commun. 2015, 6, 6888. doi: 10.1038/ncomms7888.

7.Tao, Z.; Wang, H.; Xia, Q.; Li, K.; Li, K.; Jiang, X.; Xu, G.; Wang, G.*; Ying, Z.* Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity. Hum. Mol. Genet. 2015, 24, 2426-2441.

8.Xu, G.; Deglincerti, A.; Paige, J. S.; Lundquist, M. R.; Jaffrey, S. R. Profiling lysine ubiquitination by selective enrichment of ubiquitin remnant-containing peptides. Meth. Mol. Biol. 2014, 1174, 57-71.

9.Xu, G.; Jiang, X.; Jaffrey, S. R. A mental retardation-linked nonsense mutation in cereblon is rescued by proteasome inhibition. J. Biol. Chem. 2013, 288, 29573-29585.

10. Yoon, Y.; McKenna, M. C.; Rollins, D. A.; Song, M.; Nuriel, T.; Gross, S. S.; Xu, G.; Glatt, C. E. Anxiety-associated alternative polyadenylation of the serotonin transporter mRNA confers translational regulation by hnRNPK. Proc. Natl. Acad. Sci. U.S.A. 2013, 110, 11624-11629.

11. Xu, G.; Jaffrey, S. R. Proteomic identification of protein ubiquitination events. Biotech. Genet. Eng. Rev. 2013, 29, 73-109.

12. Xu, G.; Jaffrey, S. R. Comprehensive profiling of protein ubiquitination for drug discovery. Curr. Pharm. Des. 2013, 19, 3315-3328.

13. Li H., Wan A., Xu G., Ye D. Small changes huge impact: the role of thioredoxin 1 in the regulation of apoptosis by S-nitrosylation. Acta Biochim. Biophys. Sin. 2013, 45, 153-161.

14. Xu, G.; Jaffrey, S. R. The new landscape of protein ubiquitination. Nat. Biotechnol. (News & Views) 2011, 29, 1098-1100.

15. Xu, G.; Shin, S. Y.; Jaffrey, S. R. Chemoenzymatic labeling of protein C-termini for positive selection of C-terminal peptides. ACS Chem. Biol. 2011, 6, 1015-1020.

16. Xu, G.; Jaffrey, S. R. N-CLAP: global profiling of N-termini by chemoselective labeling of the a amine of proteins. Cold Spring Harb. Protoc. 2010, 11, pdb.prot5528.

17. Xu, G.; Paige, J. S.; Jaffrey, S. R. Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling. Nat. Biotechnol. 2010, 28, 868-873.

18. Xu, G.; Shin, S. Y.; Jaffrey, S. R. Global profiling of protease cleavage sites by chemoselective labeling of protein N-termini. Proc. Natl. Acad. Sci. U.S.A. 2009, 106, 19310-19315.

19. Paige, J. S.; Xu, G.; Stancevic, B.; Jaffrey, S. R. Nitrosothiol reactivity profiling identifies S-nitrosylated proteins with unexpected stability. Chem. Biol. 2008, 15, 1307-1316.

20. Narayan, M.; Welker, E.; Zhai, H.; Han, X.; Xu, G.; McLafferty, F. W.; Scheraga, H. A. Detecting native folds in mixtures of proteins that contain disulfide bonds. Nat. Biotechnol. 2008, 26, 427-429.

21. Xu, G.; Narayan, M.; Kurinov, I.; Ripoll, D. R.; Welker, E.; Khalili, M.; Ealick, S. E.; Scheraga, H. A. A localized specific interaction alters the unfolding pathways of structural homologues. J. Am. Chem. Soc. 2006, 128, 1204-1213.

22. Xu, G.; Narayan, M.; Scheraga, H. A. The oxidative folding rate of bovine pancreatic ribonuclease is enhanced by a covalently attached oligosaccharide. Biochemistry 2005, 44, 9817-9823.

23. Xu, G.; Zhai, H.; Narayan, M.; McLafferty, F. W.; Scheraga, H. A. Simultaneous characterization of the reductive unfolding pathways of RNase B isoforms by top-down mass spectrometry. Chem. Biol. 2004, 11, 517-524.

24. Xu, G.; Narayan, M.; Welker, E.; Scheraga, H. A. Characterization of the fast-forming intermediate, des [30-75], in the reductive unfolding of onconase. Biochemistry 2004, 43, 3246-3254.

 

科研项目:

1.   国家自然科学基金面上项目:ADAM12的水解功能在小细胞肺癌增殖和转移中的分子机制研究,2015-2018

2.   国家重大科学研究计划(973计划)子课题,生物钟对下丘脑GnRH网络的调控,2014-2016(骨干成员)

3.   国家自然科学基金面上项目:来那度胺治疗多发性骨髓瘤的分子机制研究,2013-2016

 

人才项目:

2012  入选中组部第三批青年千人计划

2013  入选江苏省高层次创新创业人才(双创计划

2014  入选江苏省双创计划团队核心成员

2013  获人社部高层次留学人才回国资助

2013  江苏省“333高层次人才工程第三层次培养对象

2012  苏州工业园区第三届金鸡湖双百人才计划——海外高层次领军人才

 

 

 

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蒋小岗 副教授

 

1999年开始任教于苏州大学药理学教研室,主要参加药理学、临床药理学理论与实验教学工作。2004年毕业于苏州大学药理学专业,获硕士学位。2007年毕业于大连化学物理研究所生物分析化学专业,获博士学位。先后参加中药活性成分作用、蛋白质组学方法发展及应用等方面的研究,主持和参与科研项目4项。目前主要研究方向为分子药理学、药物蛋白质组学。

 

代表性论文:

1. Yaobin Liu, Xiangao Huang, Xian He, Yanqing Zhou, Xiaogang Jiang, Selina Chen-Kiang, Samie R Jaffrey , Guoqiang Xu . A novel effect of thalidomide and its analogs: suppression of cereblon ubiquitination enhances ubiquitin ligase function. FASEB J., 2015, 29(12), 4829-4839.

2. Guoqiang Xu, Xiaogang JiangSamie R. Jaffrey. A mental retardation-linked nonsense mutation in cereblon is rescued by proteasome inhibition. J. Biol. Chem., 2013, 288(41), 29573-29585.

3. Yan Gao, Jia Lu, Yu Zhang, Yafen Chen, Zhenlun Gu, Xiaogang Jiang . Baicalein attenuates bleomycin-induced pulmonary fibrosis in rats through inhibition of miR-21. Pulm. Pharmacol. Ther., 2013, 26(6), 649-654.

4. Xionghua Sun, Xiaogang Jiang. Combination of FASP and fully automated 2D-LC-MS/MS allows in-depth proteomic characterization of mouse zymogen granules. Biomed. Chromatogr., 2013, 27(3), 407-408.

 

研究生:

2012级:柳耀宾、段文文、隋沂言、何娴

2013级:朱莹、周艳卿、刘青、刘勋

2014级:张阳、陈苏萍

2015级:闫文文、熊礼鹏、雷青、杨静

制药工程:陈实、黄敏

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