报 告 人：Samie R. Jaffrey博士（Professor of Pharmacology, Weill Medical College of Cornell University, New York）

报告时间：2013年6月27日（星期四）15:00

报告地点：独墅湖校区二期云轩楼2301室

 

Department of Pharmacology, Weill Medical College, Cornell University, New York, NY 10021

 

Abstract: Green fluorescent protein (GFP) and its derivatives have transformed the use and analysis of proteins for diverse applications.  Like proteins, RNA has complex roles in cellular function and is increasingly used for various in vitro and in vivo applications, but a comparably robust and simple approach for fluorescently tagging RNA is lacking.  We will describe the generation of RNA aptamers that bind fluorophores resembling the fluorophore in GFP.  These RNAs activate the fluorescence of these fluorophores, resulting in a palette of RNA-fluorophore complexes that span the visible spectrum.  An RNA-fluorophore complex resembling enhanced GFP (EGFP), termed Spinach, emits a green fluorescence comparable in brightness to fluorescent proteins.  Spinach is markedly resistant to photobleaching, and Spinach fusion RNAs can be used to image RNA localization and other RNA regulatory processes in cells.  We will also discuss the use of Spinach to image other biological molecules in cells. These RNA mimics of GFP provide novel approaches to image RNA biology and other processes in cells.

 

Massachusetts Institute of Technology, M.A. & B.S. 1992 Biology

Johns Hopkins University, M.D. 1999 Medicine

Johns Hopkins University, M.D. Ph.D. 1999 Neuroscience

Johns Hopkins University, Postdoc, 1999-2001, Neuroscience/Chemical Biology

 

2001 – 2006, Assistant Professor, Department of Pharmacology, Cornell University, Weill Medical College

2006 – 2012, Associate Professor, Department of Pharmacology, Cornell University, Weill Medical College

2012 – Present, Professor, Department of Pharmacology, Cornell University, Weill Medical College

 

 

1. Jaffrey, S.R., Cohen, N.A., Rouault, T.A., Klausner, R.D., Snyder, S.H.  The iron-responsive element binding protein: A target for synaptic actions of nitric oxide.  Proc. Natl. Acad. Sci. USA., 91:12994-8, 1994.

2. Jaffrey, S.R., Snyder, S.H.  PIN: An associated protein inhibitor of neuronal nitric oxide synthase.  Science, 274:774-7, 1996.

3. Jaffrey, S.R., Snowman, A.M., Eliasson, M.J.L., Cohen, N.A., Snyder, S.H.  CAPON: A protein associated with neuronal nitric oxide synthase that regulates its interactions with PSD95.  Neuron, 20:115-24, 1998.

4. Jaffrey, S.R., Erdjument-Bromage, H., Ferris, C.D., Tempst, P., Snyder, S.H.  Protein S-nitrosylation: A physiologic signal for neuronal nitric oxide.  Nature Cell Biology, 3:193-197, 2001.

5. Jaffrey, S.R., Snyder, S.H.  The biotin switch method for the detection of S-nitrosylated proteins.  Science STKE, www.stke.org/cgi/content/full/OC_sigtrans;2001/86, 2001.

6. Jaffrey, S.R., Benfenati, F, Snowman, A.S., Snyder, S.H.  Neuronal nitric oxide synthase localization mediated by a ternary complex with synapsin and CAPON.  Proc. Natl. Acad. Sci. U.S.A., 99:3199-3204, 2002.

7. Wu, K., Hengst, U., Cox, L.J., Macosko, E.Z., Jeromin, A., Urquhart, E.R., Jaffrey, S.R.  Local translation of RhoA regulates growth cone collapse.  Nature, 436:1020-1024, 2005. PMCID: PMC1317112

8. Wu, K., Stessin, A., Kaminetsky, M., Hengst, U., Zippin, J., Buck, J., Levin, L.R., Jaffrey, S.R.  Soluble adenylyl cyclase is required for netrin-1 signaling in nerve growth cones.  Nature Neuroscience, 9:1257-1264, 2006. PMID: 16964251

9. Cox, L.J., Hengst, U., Gurskaya, N.G., Lukyanov, K.A., Jaffrey, S.R. Intra-axonal translation and retrograde trafficking of CREB promotes neuronal survival.  Nature Cell Biology, 10:149-159, 2008. PMCID: PMC3153364

10. Hengst, U., Deglincerti, A., Kim, H.J., Jeon, N.L., Jaffrey, S.R. Axonal elongation triggered by stimulus-induced local translation of a polarity complex protein. Nature Cell Biology, 11:1024-30, 2009. PMCID: PMC2724225

11. Xu, G., Shin, S.Y., Jaffrey, S.R. Global profiling of protease cleavage sites by chemoselective labeling of protein N-termini. Proc. Natl. Acad. Sci. USA., 46:19310-19315, 2009. PMCID: PMC2780798

12. Xu, G., Paige, J.S., Jaffrey, S.R. Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling, Nature Biotechnology, 28:868-873, 2010. PMCID: PMC2946519

13. Cohen, M.S., Bas Orth, C., Kim, H.J. Jeon, N.L., Jaffrey, S.R. Neurotrophin-mediated dendrite-to-nucleus signaling revealed by microfluidic compartmentalization of dendrites, Proc. Natl. Acad. Sci. USA., 108:11246-11251, 2011. PMCID: PMC3131323

14. Paige, J.S., Wu, K.Y., Jaffrey, S.R. RNA mimics of green fluorescent protein, Science, 333:642-646, 2011.

15. Xu, G., Jaffrey, S.R. The new landscape of protein ubiquitination, Nature Biotechnology, 29:1098-1100, 2011. PMCID: PMC324807

16. Paige, J.S., Ngyuen-Duc, T., Song, W., Jaffrey, S.R. Fluorescence imaging of cellular metabolites with RNA, Science, 335:1194, 2012. PMCID: PMC3303607

17. Ji, S.-J., Jaffrey, S.R. Intra-axonal translation of SMAD1/5/8 mediates retrograde regulation of trigeminal ganglia subtype specification, Neuron, 74:95-107, 2012.

18. Meyer, K.D., Saletore, Y., Zumbo, P., Elemento, O., Mason, C.E., Jaffrey, S.R.  Comprehensive analysis of mRNA methylation reveals enrichment in 3' UTRs and near stop codons, Cell, 149:1635-1646, 2012.

19. Deglincerti A., Jaffrey, S.R. Insights into the roles of local translation from the axonal transcriptome, Open Biology, 2:120079, 2012.

20. Colak, D., Ji, S.-J, Porse, B.T., Jaffrey, S.R. Regulation of axon guidance by compartmentalized nonsense-mediated mRNA decay. Cell, 153:1252-1265, 2013. doi: 10.1016/j.cell.2013.04.056. PMID: 23746841.