Shuang Yang
Shuang (Jake) Yang, Ph.D./M.D
Professor
Department of Pharmaceutical Analysis
Email: yangs2020@suda.edu.cn
Biosketch
q Education
Ph.D., University of Maryland, College Park, 2008
M.S., National University of Singapore, 2003
M.S., Shanghai Jiaotong University, China, 1997
B.S., Harbin Institute of Technology, China, 1994
q Work experience
Professor, College of Pharmaceutical Sciences, Soochow University, China, 2020-
Staff Scientist, Biological Chemistry Section, NIH/NIDCR, USA, 2019-2020
Staff Fellow, Laboratory of Bacterial Polysaccharides, FDA/CBER, USA, 2016-2019
Junior Faculty, Department of Pathology, Johns Hopkins University, USA, 2013-2016
Research Fellow, Department of Pathology, Johns Hopkins University, USA, 2011-2013
Postdoctral Fellow, Microfluic & MEMS, University of Maryland, USA, 2009-2010
Technical Support Engineer, Samsung Cheil Industries Inc., China, 2000-2001
Quality Control Manager, Shanghai Hawei New Materials Technology, 1997-1999
q Honors and awards
Jiangsu Hundred Foreigner Talent Award, China (2022)
Suzhou Industrial Park 16th Science and Technology Leadership (2022)
Jiangsu Shuangchuang Boshi Award, China (2021)
FDA CBER Scientific Achievement Award, US FDA (2018)
Young Investigator Award, 2017 ACS Pittcon, CACA (2017)
Travel Award, 2013 22nd International Symposium on Glycoconjugates (2013)
Best Poster Award. 2013 NIH/FDA Glycosciences Research Day (2013)
Presentation Award, 3rd place. The 11th World Conference of 2012 HUPO (2012)
Travel Award, The 11th World Conference of 2012 HUPO (2012)
Young Investigator Award, 2012 14th Annual Department of Pathology Research Day (2012)
Travel Award, 2011 Glycobiology Society Annual Meeting (2011)
Best Poster Award, HPLC 2008 for Innovation in Pharmaceutical Analysis (2008)
Research Interests
q Biosynthesis pathway of RNA glycosylation and its role in diseases
q Biomarker discovery of salivary fucosylation in lung cancer, abnormal fucosylation regulation mechanism of lung cancer
q Glycosylation of bronchoalveolar lavage fluid and pleural fluid in non-small cell lung disease
q Urine and duodenal body-fluid exosome-based studies on abnormal glycosylation and biomarkers in pancreatic cancer
q Regulation of serine protease activity by glycosylation in pancreatic inflammation and pancreatic cancer diagnosis
q Screening for early symptom biomarkers of human body fluids in Parkinson's and Alzheimer's
q Characterization of native glycoproteins and their Interaction with drugs
Research Fund
Mechanism of abnormal glycosylation modification on gastric cancer development caused by increased expression of β-1,4 galactosyltransferase 3 (2018-2022). (CNSF 81871955)
Glycoconjugates and cardiovascular disease (2011-2018) (P01HL107153)
Johns Hopkins proteomic innovation center in heart failure (2010-2015) (N01-HV-00240)
Clinical and analytical validation of cancer biomarkers (2010-2015) (U24CA115102)
Glycoprotein biomarkers for the early detection of aggressive prostate cancer (2012-2015) (U01CA152813)
QUANTITY: A novel analysis kit for robust drug development, clinical diagnostics, and biomarker discovery (2015-2016) (Maryland Innovation Initiative)
AGX1/2 Inhibitors as Key Modulators of the Hexosamine Biosynthetic Pathway (2014-2017) (1R21CA191715-01)
Integrating novel nutrient feeding strategies with computational glycosylation models to improve production of complex biotherapeutics from mammalian factories (2015-2018) (CBET 1512265)
Select Publications
1. J.J. Li, Z. Gao, W. Hu, Z. Liu, W. Wang, G. Xu, Y. Wang, X. Zhang, G. Zhang, M. Xue, J. Jiang, S. Yang*, “Differential expression of RNA N-linked glycosylation in pancreatic cancer cells by solid-phase capture of glycoRNAs,” submitted, 2022.
2. Q. Wang, T. Wang, W. Wu, C-Y Lin, S. Yang, G. Yang, E. Jankowska, Y. Hu, R-F. Shen, M. Betenbaugh, J.F. Cipollo, “Comprehensive N- and O-glycoproteomic analysis of multiple Chinese hamster ovary host cell lines”, Journal of Proteome Research, revision, 2022.
3. J.J. Li, B. Guo, W. Zhang, S. Yue, S. Huang, S. Gao, J. Ma, J.F Cipollo, S. Yang*, “Recent advances in demystifying O-glycosylation in health and disease”, Proteomics, under review, 2022.
4. Mingming Xu, Arthur Yang, Jun Xia, Junhong Jiang, Chun-Feng Liu, Zhenyu Ye, Junfeng Ma, Shuang Yang*, “Protein glycosylation in urine as a biomarker of disease”, Translational Research, revision, 2022.
5. J.J. Li, J. Zhang, M. Wu, Z. Yang, S. Yue, W. Zhou, C. Gui, H. Zhang, S. Li, P.G. Wang, S. Yang*, “Recent advances in glycopeptide enrichment methods for the analysis of protein glycosylation using mass spectrometry”, Journal of Separation Science, accepted, 2022.
6. M. Xu, H. Jin, Y. Han, Z. Wu, J. Chen, C. Mao, X. Zhang, P. Hao, C. Liu, S. Yang*, “Mass spectrometry-based analysis of serum N-glycosylation changes in patients with Parkinson’s disease,” ACS Chemical Neuroscience 2022, 13(12), 1719-1726.
7. Z. Gao, Z. Wu, Y. Han, X. Zhang, P. Hao, M. Xu, S. Huang, S. Li, J. Xia, J. Jiang*, and S. Yang*, “Aberrant fucosylation of saliva glycoprotein defining lung adenocarcinomas malignancy,” ACS Omega 2022, 7, 17894-17906.
8. Z. Gao, M. Xu, S. Yue, S. Huang, J. Xia, J. Jiang, and S. Yang*, Abnormal sialylation and fucosylation of saliva glycoproteins: characteristics of lung cancer-specific biomarkers, Current Research in Pharmacology and Drug Discovery 2022, 3, 100079, 1-13.
9. Q. Wang, T. Wang, R. Zhang, S. Yang, K. McFarland, C-y. Chung, H. Jia, L. Wang, J.F. Cipollo, M.J. Betenbaugh*, “The interplay of protein engineering and glycoengineering to fine-tune antibody glycosylation and its impact on effector functions,” Biotechnology and Bioengineering 2021, 119, 102-117.
10. Q. Wang, Y. Wang, S. Yang, C. Lin, L. Aliyu, Y. Chen, L. Parsons, Y. Tian, H. Jia, A. Pekosz, M.J. Betenbaugh, J.F. Cipollo, “A Linkage-specific sialic acid labeling strategy reveals different site-specific glycosylation patterns in SARS-CoV-2 spike protein produced in CHO and HEK cell substrates,” Frontiers in Chemistry 2021, 9:735558,1-16.
11. Y. Wang, Q. Wang, Z. Wu, W. Hu, P. Hao, and S. Yang*, “Impact of expressing cells on glycosite occupancy and glycan of SARS-CoV-2 spike glycoprotein,” ACS Omega 2021, 6(24), 15988-15999.
12. U.H. Mortensen*, D.C. Anyaogu, A.H. Hansen, J.B. Hoof, N.I. Majewska, J.T. Paul, K.R. Nielsen, T.J. Hobley, S. Yang, H. Zhang, and M. Betenbaugh, “Glycoengineering of aspergillus nidulans to produce precursors for humanized N-glycan structures,” Metabolic Engineering 2021, 67, 153-163.
13. M. Xu, W. Hu, Z. Liu, J. Xia, S. Chen, P.G. Wang, S. Yang*, “Glycoproteomic bioanalysis of exosomes by LC-MS to early diagnose pancreatic cancer,” Bioanalysis 2021, 13(11), 861-864.
14. S. Yang*, J. Xia, Z. Yang, M. Xu, S. Li, “Lung cancer molecular mutations and abnormal glycosylation as biomarkers for early diagnosis”, Cancer Treatment and Research Communications 2021, 21(7), 100311, 1-11.
15. M. Xu, M. Zhou, S. Li, X. Zhen, and S. Yang*, “Glycoproteins as diagnostic and prognostic biomarkers for neurodegenerative diseases: a glycoproteomic approach,” Journal of Neuroscience Research 2021, 99(5), 1308-1324.
16. H. Yu, J. Wang, Z. Tang, X. Li, M. Yin, F. Zhang, J. Shu, W. Chen, S. Yang*, and Z. Li*, “Integrated glycomics strategy for evaluation of glycosylation alterations in salivary proteins associated with type 2 diabetes mellitus,” RSC Advances 2020, 10, 39739-39752.
17. S. Yang, Y. Wang, M. Mann, Q. Wang, E. Tian, L. Zhang, J.F. Cipollo, K.G.T. Hagen, and L.A. Tabak*, “Improved online LC-MS/MS identification of O-glycosites by EThcD fragmentation, chemoenzymatic reaction, and SPE enrichment,” Glycoconjugate Journal 2020, 38(2), 145-156.
18. S. Yang*, W. Wu, R-f Shen, L. Parsons, and J.F. Cipollo*, “Optimization of O-GIG for O-Glycopeptide Characterization with Sialic Acid Linkage Determination,” Analytical Chemistry 2020, 92(16), 10946-10951.
19. Q. Wang, T. Wang, S. Yang, S. Sha, W.W. Wu, Y. Chen, J.T. Paul, R-f. Shen, J.F. Cipollo, and M.J. Betenbaugh*, “Metabolic Engineering Challenges to extending N-glycan pathways in Chinese hamster ovary cells,” Metabolic Engineering, 2020, 61, 301-314.
20. N. de Haan*, S. Yang, J.F. Cipollo, and M. Wuhrer, “Glycomics studies using sialic acid derivatization and mass spectrometry,” Nature Reviews Chemistry 2020, 4, 229-242.
21. M. Lorna A. De Leoz et al., “NIST interlaboratory study on glycosylation analysis of monoclonal antibodies: comparison of results from diverse analytical methods,” Molecular & Cellular Proteomics 2020, 19(1), 11-30.
22. C. Chung, Q. Wang, S. Yang, S. Chough, J.F. Cipollo, J.P. Balthasa, and M.J. Betenbaugh*, “The impact of sialylation linkage-type on the pharmacokinetics of recombinant butyrylcholinesterases,” Biotechnology and Bioengineering 2019, 117(1), 157-166.
23. H. Wan*, J. Gao, H. Yang, S. Yang, R. Harvey et al., “The neuraminidase of A(H3N2) influenza viruses circulating since 2016 is antigenically distinct from the A/Hong Kong/4801/2014 vaccine strain,” Nature Microbiology 2019, 4, 2216-2225.
24. S. Yang, Z. Yang, P. Wang*, “Bioanalysis for precision medicine”, Bioanalysis 2019, 11(11), 1039-1043.
25. S. Yang*, P. Onigman, W. Wu, J. Sjogren, H. Nyhlen, R-F. Shen, and J. Cipollo*, “Deciphering protein O-glycosylation: solid-phase chemoenzymatic cleavage and enrichment,” Analytical Chemistry 2018, 90(13), 8261-8269 (ACS Editor’s Choice).
26. S. Yang*, W. Wu, R-F. Shen, M. Bern, and J. Cipollo*, “Identification of sialic acid linkages on intact glycopeptides via differential chemical modification using intactGIG-HILIC,” Journal of the American Society for Mass Spectrometry 2018, 29(6), 1273-1283.
27. S. Yang*, S. Chatterjee*, and J. Cipollo, “The glycoproteomics-mass spectrometry for studying cardiac hypertrophy and heart failure,” Proteomics: Clinical Applications 2018, 12(5), 1870042, 1-13.
28. N. Hoti, S. Yang, Y. Hu, P. Shah, M. Haffner, and H. Zhang*, “Overexpression of α (1,6) fucosyltransferase in the development of castration-resistant prostate cancer cells,” Prostate Cancer and Prostatic Diseases 2018, 21(1), 137, 1-10.
29. W. Zhou, S. Yang, P.G. Wang*, “Matrix effects and matrix effect factors,” Bioanalysis 2017, 9(23), 1839-1844.
30. S. Yang*, D. Clark, Y. Liu, S. Li, H. Zhang, “High-throughput analysis of N-glycans using AutoTip via glycoprotein immobilization for glycan extract,” Scientific Reports 2017,7(1), 10216, 1-11.
31. S. Yang*, L. Chen, D.W. Chen, Q. Li, and H. Zhang, “Protein signatures of molecular pathways in non-small cell lung carcinoma (NSCLC): comparison of glycoproteomics and global proteomics,” Clinical Proteomics 2017,14(1), 31, 1-15.
32. S. Yang, E. Jankowska, M. Kosikova, H. Xie, and J. Cipollo*, “Solid-Phase Chemical Modification for Sialic Acid Linkage Analysis: Application to Glycoproteins of Host Cells Used in Influenza Virus Propagation,” Analytical Chemistry 2017, 89(17), 9508-9517.
33. N. Hoti*, S. Yang, P. Aiyetan, B. Kumar, Y. Hu, D. Clark, A.U. Eroglu, P. Shah, T. Johnson, W.H. Chowdery, H. Zhang, and R. Rodriguez, “Overexpression of Exportin-5 Overrides the Inhibitory Effect of miRNAs Regulation Control and Stabilize Proteins via Posttranslation Modifications in Prostate Cancer,” Neoplasia 2017, 19(10), 817-829.
34. S. Yang* and P. Wang, “Method development of glycoprotein biomarkers for cancers,” Bioanalysis 2017; 9(12):903-906.
35. C. Chung, Q. Wang, S. Yang, S.A. Ponce, B.J. Kirsch, H. Hang, M.J. Betenbaugh*, “Combinatorial genome and protein engineering yields monoclonal antibodies with hypergalactosylation from CHO cells,” Biotechnology and Bioengineering 2017, 114(12), 2848-2856.
36. S. Yang*, L. Zhang, S. Thomas, Y. Hu, S. Li, J. Cipollo, and H. Zhang, “Modification of sialic acids on solid-phase: accurate characterization of protein sialylation,” Analytical Chemistry 2017, 89(12), 6330-6335.
37. S. Yang*, Y. Hu, L. Sokoll, and H. Zhang*, “Simultaneous quantification of N- and O-glycans using a solid-phase method”, Nature Protocols 2017, 12(6), 1229-1244.
38. S. Yang*, N. Hoti, W. Yang, Y. Liu, S. Li, and H. Zhang*, “Simultaneous analyses of N-linked and O-linked glycans using solid-phase chemoenzymatic method,” Clinical Proteomics 2016, 14(3), 1-11.
39. C. Chung, Q. Wang, S. Yang, B. Yin, H. Zhang, M. Betenbaugh*, “Integrated Genome and Protein Editing Swaps α‐2, 6 Sialylation for α‐2, 3 Sialic Acid on Recombinant Antibodies from CHO,” Biotechnology Journal 2017, 12(2), 1600502, 1-11.
40. N. Hoti*, P. Shah, Y. Hu, S. Yang, and H. Zhang, “Proteomics analyses of prostate cancer cells reveals cellular pathways associated with androgen resistance,” Proteomics 2017, 17, 6, 1-10.
41. D. Do, S. Yang, X. Yao, R.G. Hamilton, J.T. Schroeder, P. Gao*, “N-glycan in cockroach allergen regulates human basophil function,” Immunity, Inflammation and Disease 2017, 5(4), 386-399.
42. X. Jia, J. Chen, S. Sun, W. Yang, S. Yang, P. Shah, N. Hoti, B. Veltri, and H. Zhang*, “Detection of aggressive prostate cancer-associated glycoproteins in urine using glycoproteomics and mass spectrometry,” Proteomics 2016, 16(23), 2928.
43. S. Yang*, A. Rubin, S. Toghi Eshghi, and H. Zhang, “Chemoenzymatic method for glycomics: Isolation, identification, and quantitation,” Proteomics 2016, 16, 241-256.
44. S. Sun, P. Shah, S. Toghi Eshghi, W. Yang, N. Trikannad, S. Yang, L. Chen, P. Aiyetan, N. Hoti, Z. Zhang, D.W. Chan, and H. Zhang*, “Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides,” Nature Biotechnology 2016, 34(1), 84-88.