高通量测序技术与基因组学研究

报告题目：高通量测序技术与基因组学研究
报 告 人：高汉林博士
报告时间：2011年10月24日（周一）下午2:00
报告地点：独墅湖校区炳麟图书馆720室

高汉林博士简介

Education/Training
The Ohio State University, Columbus, Ohio    Ph.D.     03/2001 Medical Genetics
Harvard Medical School, Boston , MA         Postdoc. 07/2004 Molecular Genetics
Harvard Medical School, Boston , MA        ABMG   07/2004 Clinical Molecular Genetics

Positions and Employment
2001-2004    Research Fellow, Harvard Medical School, Boston, MA     
2004-2008   Co-Director, Clinical Molecular Diagnostics Laboratory, City of Hope Medical Center
2004-       Assistant research professor, City of Hope Medical Center
2008-2009   Associated Direcotor, DNA sequencing/Solexa Core lab, Beckman Research Institute of City of Hope   
2009-      Co-Director, Function genome/genome sequencing Core lab, Beckman Research Institute of City of Hope

Awards and Honors
2002-2003           Tosteson Postdoctoral Fellowship
1996-2000           Bennett Fellowship, The Ohio State University
1996                    University Fellowship, The Ohio State University
1993                    Guang Hua Prize, Beijing Medical University
1986-1990           Fellowship, Neimenggumeng Medical College

Selected peer-reviewed publications
1.Gao H, Huang KC, Yamasaki EF, Chan KK, Chohan L, Snapka RM.. XK469, selective topoisomerase II b poison. Proc. Natl. Acad. Sci. U.S.A. 1999;96:12168-12173. PMCID: PMC18430
2.Gao H, Yamasaki EF, Chan KK, Shen LL, Snapka RM.  Chloroquinoxaline sulfoamide is a topoisomerase II a/b Poison. Cancer Research, 2000, 60:5937-5940 PMID: 11085507
3.Gao H, Yamasaki EF, Chan KK, Shen LL, Snapka RM. DNA sequence specificity for topoisomerase II poisoning by the quinoxaline anticancer drugs XK469 and CQS. Mol Pharmacol. 2003; 63(6):1382-8. PMID: 12761349
4. Huang KC, Gao H(co-first author), Yamasaki EF, Grabowski DR, Liu S, Shen LL, Chan KK, Ganapathi R, Snapka RM.  Topoisomerase II poisoning by ICRF-193. J Biol Chem. 2001; 276(48):44488-94. PMID:11577077
5. Snapka RM, Gao H, Grabowski DR, Brill D, Chan KK, Li L, Li GC, Ganapathi R.  Cytotoxic mechanism of XK469: resistance of topoisomerase IIb knockout cells and inhibition of topoisomerase. Biochem Biophys Res Commun. 2001; 280(4):1155-60.
6 .Gao H, Boustany RM, Espinola JA, Cotman SL, Srinidhi L, Antonellis KA, Gillis T, Qin X, Liu S, Donahue LR, Bronson RT, Faust JR, Stout D, Haines JL, Lerner TJ, MacDonald ME. Mutations in a novel cln6-encoded transmembrane protein cause variant neuronal ceroid lipofuscinoses in man and mouse. Am J Hum Genet. 2002; 70(2):324-35 PMCID: PMC384912
7. Fossale E, Wolf P, Espinola JA, Lubicz-Nawrocka T, Teed AM, Gao H, et al .  Membrane trafficking and mitochondrial abnormalities precede subunit c deposition in a cerebellar cell model of juvenile neuronal ceroid lipofuscinosis. BMC Neurosci. 2004 Dec 10;5(1):57. PMCID: PMC539297
8.Zhang,J；Zhou,C；Yin,Y；Chen,L；Pardinas,J.R；Liu,T；Gao,H；Saldivar-J.,S；Li,K  Detection of Known Mutations with DNA Polymerases: Its Pharmacogenetic Applications. Current Pharmacogenomics, 2006, 4, 121-131
9  .Zeng WQ, Gao H, Brueton L, Hutchin T, Gray G, Chakrapani A, Olpin S, Shih VE. Fumarase deficiency caused by homozygous of P131R mutation on partial uniparental isodisomy of Chromosome 1. Am J Med Genet A. 2006;140(9):1004-9. PMID:16575891
10.Adegbola A, Gao H, Sommer S, Browning M.. A Novel Mutation in JARID1C / SMCX Associated with Autism Spectrum Disorder (ASD). Am J Med Genet A 2008 Feb 15;146(4):505-11. PMID:18203167
11.Liang S, Bass HN, Gao H, Astbury C, Jamehdor MR, Qu Y. A Pseudo-full Mutation in Fragile X Assay Resulted from a Novel Base Change Abolishing an EcoRI Restriction Site Journal of Molecular Diagnostics, 2008;. 10 (5):469-474
12 .Weng L, Wu X, Gao  H(co-first author), Mu B, Li X, Wang J, Guo C,  Jin J, Chen Z, Covarrubias M, Yuan YC, Weiss LM, Wu H. MicroRNA profiling of clear cell renal cell carcinoma by whole genome small RNA deep sequencing of paired frozen and formalin-fixed paraffin-embedded tissue specimens. J Pathol. 2010 Sep;222(1):41-51. PMID: 20593407
13 .Tao R, Wei D, Gao H, Liu Y, Depinho RA, Dong XC. Hepatic FoxOs regulate lipid metabolism via modulation of expression of the nicotinamide phosphoribosyltransferase gene. J Biol Chem. 2011 Apr 22;286(16):14681-90. PMCID: PMC3077665
14.Duldulao MP, Lee W, Le M, Chen Z, Li W, Wang J, Gao H, Li H, Kim J, Garcia-Aguilar J, Gene Expression Variations in Microsatellite Stable and Unstable Colon Cancer Cells J Surg Res. 2011 Jul 7. [Epub ahead of print] PMID: 21816436
15 .Sun G, Wu X,  Wang J, Li H,  Gao H, Rossi J, Yen Y True” Small RNAome Revealed by a Bias-Reducing Strategy in Profiling Small RNAs Using Solexa.  RNA  (2011 in press)

Research Support.
5R37ES006070-19       PFEIFER (PI)                    04/28/10-04/30/14
UV DAMAGE, REPAIR, AND MUTAGENESIS
The goal of this study is the molecular mechanisms of UV damage, repair and mutagenesis. CpG-methylated mutation will be studied using Next Generation DNA sequencing technology.

5R01CA084469-11      PFEIFER (PI)                     03/01/10-02/28/14
CPG METHYLATION AND MUTATION
The goal of this study is Transcriptional gene silencing by hypermethylation of CpG islands spanning the promoter regions of genes. CpG-methylated mutation will be studied using Next Generation DNA sequencing technology. 

CBCRP IDEA, Emily Wang                             7/1/2010-12/31/2011
Identify Blood-based MiRNA Markers of NCT Response in Breast Cancer.

1R01MH094599               LU (PI)                                    6/15/11-3/31-2016
EPIGENOMIC MODIFICATIONS IN MAMMALIAN NEUROGENESIS
The goal of this study is to understand how epigenomic modifications in neural stem/progenitor cells contribute to the control of a dynamic balance between the state of self-renewal and differentiation.  

5R01AG036041-03         PFEIFER (PI)                            9/15/2009-8/31/2014
AGING AND THE UNSTABLE EPIGENOME
 To study epigenetic changes are a hallmark of the aging process.  

1R01NS075393-01   LU (PI)                                      5/1/2011-2/29/2016
MOLECULAR GENETIC ANALYSIS OF MAMMALIAN NEURONAL DIFFERENTIATION
Our proposed study on identification and characterization of causal factors in neural progenitor cell regulation  to provide insight into how self-renewal and differentiation are regulated, which has direct implication on future regenerative medicine and is also useful for understanding of tumor development.

1R01HL106089-01   NATARAJAN (PI)                     1/1/2011-12/31/2015
TRANSCRIPTIONAL REGULATION BY ANGIOTENSIN II IN VASCULAR SMOOTH MUSCLE CELLS
Our goal is to evaluate such epigenetic and miR mechanisms in Ang II actions in order to unravel new therapeutic targets.